A Novel Agent for Oral Surface Conditioning to Control Bacterial Adhesion

 描述（由申请人提供）：生物膜是病原体的储存库，几乎遍布所有表面，包括口腔中的表面； dental plaque is a complex biofilm.口腔生物膜引起牙周问题，通常是由形成生物膜的细菌产生酸作用和牙齿脱矿质引起的；牙菌斑与许多严重的系统问题有关，如果不加以治疗和控制，可能会导致许多严重的系统问题。特别是，它们对免疫功能低下的患者造成严重的健康危害。生物膜的形成和在表面的定植始于生物膜形成细菌的粘附；细菌最初附着在支撑表面对于它们的生存能力、成功定殖以及由此产生的感染至关重要。控制牙齿和相关表面上牙菌斑形成的合理方法是定期清洁 用一些能抵抗生物膜形成细菌附着的东西来调节它们。 该第一期 SBIR 提议开发一种用于牙齿和相关表面的调理剂，并优化其化学结构（相对于其抑制细菌生物膜附着的能力）。建议将优化的调理剂用于牙齿清洁制剂（例如牙膏、漱口水或清漆）或清洁后冲洗制剂中。在冲洗期间（与日常牙齿清洁一样频繁），该产品将对口腔表面进行表面改性，使得改性的牙齿和相关表面将控制生物膜形成细菌的附着。 为了证明抑制细菌粘附在用作牙科和相关表面模型的生物材料上的可行性，旨在制备具有不同程度的疏水性/亲水性的调理生物分子，并将其涂覆在模型牙科相关生物材料上，以量化涂覆表面上的细菌粘附和产酸作用，并在连续流培养生物膜模型中生长生物膜，并与相关的未涂覆对照表面上的生物膜进行比较。还建议优化涂层材料的分子结构，以最好地抑制细菌粘附、控制乳酸形成及其活力。 之后 在第一阶段研究完成后，许多使用本提案中确定的优化生物分子的表面调理配方可能会在稍后的第二阶段部分进一步开发 项目。迄今为止，在宿主感染后控制感染方面已经进行了大量的研究，但结果相当无效，因为几乎所有致病细菌仍然受到生物膜的保护，并对治疗药物表现出耐药性。因此，在感染站稳脚跟之前，需要大量的科学关注来控制生物膜本身的形成过程。

 DESCRIPTION (provided by applicant): Biofilms are the reservoirs for pathogens and colonize almost all surfaces, including those in the oral cavity; dental plaque is a complex biofilm. Oral biofilms cause periodontal problems commonly induced by acidogenesis and tooth demineralization by biofilm forming bacteria; dental plaque has been associated to and may lead to many serious systemic problems if are it not treated and controlled. In particular, they pose serious health evils to immuno- compromised patients. Biofilm formation and colonization on a surface starts with is the adhesion of biofilm forming bacteria; initial bacterial attachment o the support surface is vital for their viability and successful colonization and the resultant infection. A logical way to control plaque formation on dental and related surfaces is to regularly condition them with something that would resist the attachment of biofilm forming bacteria. This Phase-I SBIR proposes to develop a conditioning agent for dental and related surfaces and the optimization of its chemical structure relative to its ability to inhibit attachment of biofilm foring bacteria. The optimized conditioning agent is proposed to be used in dental cleaning formulations (for example, tooth paste, mouth rinse or varnish) or in a post-cleaning rinse formulation. During the rinses (as frequent as daily dental cleaning), the product will perform surface modification of oral surfaces such that the modified dental and related surfaces would control the attachment of biofilm forming bacteria. To prove the feasibility of inhibition of bacterial adhesion on biomaterials used as the model for dental and related surfaces, it is intended to prepare the proposed conditioning biomolecules with varying degree of hydrophobicity/hydrophilicity and coat them on model dental related biomaterials to quantify the bacterial adherence and acidogenesis on coated surfaces and also to grow biofilm in a continuous flow culture biofilm model and compare to that on the related uncoated control surfaces. It is also proposed to optimize the molecular structure of coating material for the best inhibition of bacterial adherence, control of lactic acid formation and their viability. After the completion of this phase-I study, many surface conditioning formulations using the optimized biomolecules identified in this proposal may be further developed later in the phase-II part of the project. A huge burden of research has been carried out so far on infection control after the infection is established in host, but the results are quite ineffective as almost all disease causig bacteria remain protected in biofilms and show resistance to therapeutic agents. Therefore, a significant scientific attention is needed to control the process of formation of biofilm itself, before the infection could set firm footing.