Investigation of Myo-Inositol Oxygenase

肌醇加氧酶的研究

• 批准号: 9633445
• 负责人: Eugene Mueller
• 金额: $ 3.2万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9633445&HistoricalAwards=false
• 关键词: Investigation; Myo; Inositol; Oxygenase

This starter grant award to the University of Delaware supports the research of Professor Eugene G. Mueller. The research is an investigation of the mechanism and structure of the enzyme myo-inositol oxygenase, whose catalytic function arises from a non-heme iron in a protein environment. Techniques employed include nuclear magnetic resonance and Moessbauer spectroscopies; rapid freeze quench methods; site-directed mutagenesis; and steady-state and rapid kinetic studies including substrate analogs, inhibitors, and the determination of isotope effects. In addition to the novel chemistry to emerge from these studies, the enzyme myo-inositol oxygenase is of physiological interest. Diabetic animals show a marked decrease in its acitivity, and inhibition of the enzyme leads to rapid kidney failure. Inositol phosphates play a key role in cellular signaling, errors in which mediate oncogenesis. Thus, non-heme, iron-containing enzymes are vital in life-sustaining biological processes.

这个启动赠款奖给特拉华州大学支持 尤金G.穆勒 这项研究是一项 肌醇酶的结构和作用机理的研究 加氧酶，其催化功能来自非血红素铁， 蛋白质环境 所采用的技术包括核磁共振 共振和穆斯堡尔谱;快速冷冻淬火法; 定点诱变;稳态和快速动力学研究 包括底物类似物、抑制剂和同位素的测定 方面的影响. 除了从这些研究中出现的新化学， 酶肌醇加氧酶具有生理学意义。 糖尿病 动物显示其活性显著降低， 酶会导致快速肾衰竭 肌醇磷酸盐起着关键作用 在细胞信号传导中的作用，介导肿瘤发生的错误。 因此，在本发明中， 非血红素，含铁的酶是维持生命的生物至关重要的， 流程.