Capillary Electrophoresis in the Analytical and Physical Chemistry Laboratories

自20世纪80年代初引入以来，毛细管电泳（CE）已发展成为生物大分子高分辨率分离的重要工具。持续的方法开发已经将应用扩展到各种各样的各种大小的带电和中性化合物。为了为将来的就业和高等教育做好准备，学生应该对这种工具方法有更多的教科书知识。为了提供这种经验，涉及使用CE系统的实验正在为三个高级化学实验室开发。仪器分析专业的学生正在使用反相液相色谱和毛细管电泳分析一种商业感冒/鼻窦药物。他们正在观察两种分离方法的差异以及洗脱液特性和pH值的重要性。标准物理化学实验室的学生正在测量电泳过程的几个基本物理参数。一个新的物理化学实验室，强调生物和合成大分子的性质正在提供作为标准课程的替代。选择该选项的学生使用CE来跟踪酶催化反应的动力学。

Since its introduction in the early 1980's, capillary electrophoresis (CE) has grown to become an essential tool for high resolution separations of biological macromolecules. Continuing method development has extended applications to a wide variety of charged and neutral compounds of all sizes. To be prepared for future employment and advanced schooling, students should have more than textbook knowledge of this instrumental method. To provide this experience, experiments that involve use of a CE system are being developed for three upper-level chemistry laboratories. Students in instrumental analysis are analyzing a commercial cold/sinus remedy using both reversed-phase liquid chromatography and capillary electrophoresis. They are observing the differences in the two separation methods and the importance of eluent character and pH. Students in the standard physical chemistry laboratory are measuring several physical parameters fundamental to the electrophoresis process. A new physical chemistry laboratory, emphasizing the properties of biological and synthetic macromolecules is being offered as an alternate to the standard course. Students who choose that option use CE to follow the kinetics of an enzyme-catalyzed reaction.