SBIR Phase I: Novel Chemical Synthesis of Ribo Nucleic Acid (RNA) Oligonucleotides

SBIR 第一阶段：核糖核酸 (RNA) 寡核苷酸的新型化学合成

• 批准号: 9660058
• 负责人: Stephen Scaringe
• 金额: $ 7.5万
• 依托单位: DHARMACON INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9660058&HistoricalAwards=false
• 关键词: SBIR; Phase; Novel; Chemical; Synthesis

This project will evaluate the quality and utility of RNA oligonucleotides synthesized using a novel chemical synthesis scheme. The growing interest in RNA structural components, ribazymes and other RNA functions has generated a need for reliable methods of synthesizing small RNA oligonucleotides (50 nucleotides). Although RNA synthesis can be accomplished for some applications via either biochemical methods, em. transcription, or chemically, e.g. 5'-dimethoxytrityl-2'-silyl chemistry, there is a definite need for improved RNA synthesis methods. Final processing of synthesized RNA must be done under mild, sterile conditions with minimal handling. Our final processing is accomplished under mild aqueous conditions, pH 3, 55 ÝC for 10 minutes. The purpose of this study is twofold: (1) establish the general utility of this chemistry by synthesizing a variety of RNA oligonucleotides to be evaluated by several collaborators, (2) confirm the RNA quality by using a broad spectrum of chemical and biochemical assays. The market for oligonucleotides should grow significantly to a multi-million dollar level if this cost effective route to these materials can be developed.

该项目将评估使用新的化学合成方案合成的RNA寡核苷酸的质量和效用。对RNA结构组分、核酶和其他RNA功能的兴趣日益增长，产生了对合成小RNA寡核苷酸（50个核苷酸）的可靠方法的需求。虽然RNA合成可以通过任何一种生物化学方法在某些应用中完成，但在某些应用中，RNA合成可以通过任何一种生物化学方法完成。由于RNA合成方法在转录或化学上，例如5 ′-二甲氧基三苯甲基-2 ′-甲硅烷基化学上的应用，因此确实需要改进的RNA合成方法。 合成RNA的最终加工必须在温和、无菌的条件下进行，操作量最少。我们的最终加工是在温和的水溶液条件下完成的，pH值为3，55 ℃，10分钟。本研究的目的是双重的：（1）通过合成各种RNA寡核苷酸来建立这种化学的一般效用，由几个合作者进行评估，（2）通过使用广谱化学和生物化学测定来确认RNA质量。 寡核苷酸市场将显著增长到数百万美元的水平，如果这种具有成本效益的路线，这些材料可以开发。