SBIR Phase II: Novel Chemical Synthesis of Ribo Nucleic Acid (RNA) Oligonucleotides

*** 9801382这个小企业创新研究（SBIR）二期项目将使定制RNA合成能够负担得起，及时，并通过纳入所需的修饰而更强大。I期实验结果表明，新的5'-硅基-2'-正位酯RNA合成化学方法能够以高产量和前所未有的质量生产真正的RNA寡核苷酸。然而，如果这种化学反应被限制在全核糖寡核苷酸的合成中，它的应用将受到限制。市场研究和正在进行的协商突出表明，还需要作出修改。目前商业化的RNA合成化学方法允许结合修饰，但只有化学家和其他知识渊博的专家似乎在这些替代方法上取得了重大成功。RNA合成对生物学家来说并不容易。第二阶段目标的实现将导致5'-硅基-2'-正构酯化学，为所有生物学家提供必要的技术飞跃，使定制RNA合成随时可用。这将需要合并所需的修改和减少合成成本和周转时间。其结果将是整个科学界的定制RNA合成的可访问性，用于广泛的应用。项目的成功将满足研究界对可负担的、可靠的、定制的RNA合成的需求。＊＊＊

*** 9801382 This Small Business Innovation Research (SBIR) Phase II project will enable custom RNA Synthesis to be affordable, timely, and more powerful through the incorporation of desired modifications. Phase I results demonstrated that novel 5'-silyl-2'-orthoester RNA synthesis chemistry yields authentic RNA oligonucleotides in high yield and of unprecedented quality. However, the utility of this chemistry would be limited if it was restricted to the synthesis of all-ribo oligonucleotides. Market research and ongoing consultations have highlighted the additional need to include modifications. Current commercial RNA synthesis chemistries allow incorporation of modifications, but only chemists and other knowledgeable specialists appear to have significant success with these alternatives. RNA synthesis is not readily accessible to biologists. The achievement of the Phase II objectives will result in 5'-silyl-2'-orthoester chemistry providing the technological leap necessary to make custom RNA synthesis readily available to all biologists. This will require both incorporating desired modifications and decreasing synthesis costs and turnaround times. The result will be the ready accessibility of custom RNA synthesis to the entire scientific community for a wide range of applications. Project success will satisfy the needs of the research community for affordable, reliable, custom synthesis of RNA toward numerous applications. ***