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SBIR Phase I: Polystyrene Supports for Large Scale and Long RNA Synthesis

SBIR 第一阶段：聚苯乙烯支持大规模和长 RNA 合成

基本信息

批准号：
9760309
负责人：
Stephen Scaringe
金额：
$ 10万
依托单位：
DHARMACON INC
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1998
资助国家：
美国
起止时间：
1998-01-01 至 1998-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9760309&HistoricalAwards=false
关键词：
SBIR Phase Polystyrene Supports Large

项目摘要

*** 9760309 Scaringe This Small Business Innovation Research Phase I project will evaluate the effectiveness of non-porous polystyrene beads derivatized with branched linkers to yield a high loaded support to be used in conjunction with an efficient RNA synthesis chemistry. This support will enable (1) large scale RNA syntheses and (2) syntheses of RNA up to 120 bases.The company is successfully developing an RNA chemistry in which each nucleotide couples in less than 90 seconds with yields in excess of 99%. 36-Nucleotide RNA oligonucleotides are functional sites on the external and internal surfaces of porous polystyrene beads. Unfortunately, the pores constrain the amount and length of RNA that can be synthesized. Large syntheses and long sequences are unfeasible with this chemistry on porous support. Development of a suitable non-porous bead is proposed.. Oligonucleotides would grow out into solution without limitations of length. Branched linkers would multiply functional surface sites for large syntheses. These supports will be evaluated with the existing RNA chemistry to synthesize larger scales and longer sequences. Project success will satisfy research and pharmaceutical market needs for large scale RNA syntheses and routine synthesis of RNA less than or equivalent to 120 bases long. These more powerful RNA synthesis capabilities will help the biotechnology and health care industries in investigating RNA-based therapeutics. ***

* 9760309 Scaringe 这个小企业创新研究第一阶段项目将评估用分支连接体衍生的无孔聚苯乙烯珠的有效性，以产生高负载的支持物，与有效的RNA合成化学结合使用。这种支持将使（1）大规模RNA合成和（2）高达120个碱基的RNA合成成为可能。该公司正在成功开发一种RNA化学，其中每个核苷酸在不到90秒的时间内偶联，产率超过99%。36-核苷酸RNA寡核苷酸是多孔聚苯乙烯珠的外表面和内表面上的功能位点。不幸的是，这些孔限制了可以合成的RNA的数量和长度。在多孔载体上进行大规模合成和长序列是不可行的。提出了一种合适的无孔珠的开发。寡核苷酸将生长到溶液中而没有长度限制。分支连接体将增加大型合成的功能表面位点。这些支持物将用现有的RNA化学进行评价，以合成更大规模和更长的序列。项目的成功将满足研究和制药市场对大规模RNA合成和常规合成小于或等于120个碱基长度的RNA的需求。这些更强大的RNA合成能力将有助于生物技术和医疗保健行业研究基于RNA的疗法。 ***