Identification of candidate genes responsible for preformed and inducible defence response in oaks against herbivorous insects

鉴定负责橡树针对草食性昆虫的预形成和诱导防御反应的候选基因

• 批准号: 112762180
• 负责人: Privatdozent Dr. Matthias Fladung
• 金额: --
• 依托单位: Institut für Forstgenetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/112762180?language=en
• 关键词: Identification; candidate; genes; responsible; preformed

The defoliation of oaks is an urgent problem for forestry in Central Europe. During the last outbreak of the green oak leaf roller (Tortrix viridana) in 2003-2005, we observed fundamental differences in the defoliation level of individual Quercus robur trees in Germany. Some of the trees seem to be somehow “tolerant” (T oaks) against the insects grub while some seem to be conspicuously “susceptible” (S oaks). Within this study we aim to identify the underlying molecular and biochemical mechanisms in oaks responsible for the behavioural preference of Tortrix viridana. By means of combined feeding choice experiments and biochemical analysis of preformed and induced defence mechanisms in T and S oak phenotypes, we will identify the metabolic/chemical basis of the observed differences as prerequisite for the selection of candidate genes expressed in tolerant trees, but not in susceptible ones or vice versa after insects feeding. We will identify functional genes involved in the defence response (i.e. phenolic compounds and plant volatiles) of oak (Q. robur) trees via microarray analysis in already identified T and S oak phenotypes in a research area in North Rhine-Westphalia (Western Germany). This approach will give us a deep insight into the functional genomics of Q. robur relating to the feeding of herbivorous insects. With the identification of molecular and biochemical markers of “Tortrix tolerance” in oaks we can contribute to decision support in sustainable forest management.

橡树落叶是中欧林业面临的一个紧迫问题。在2003-2005年的绿色橡树卷叶蛾（绿色卷叶蛾）的最后一次爆发期间，我们观察到在德国的个体栎树的落叶水平的根本差异。有些树似乎对昆虫幼虫有某种“容忍”（T橡树），而有些树似乎明显“敏感”（S橡树）。在这项研究中，我们的目标是确定潜在的分子和生化机制，橡树负责的行为偏好的Toralumviridana。通过联合喂养选择实验和生化分析的预成型和诱导防御机制的T和S橡树表型，我们将确定所观察到的差异的代谢/化学基础的先决条件选择的候选基因表达在耐受树，但不是在敏感的，反之亦然昆虫喂养后。我们将确定功能基因参与防御反应（即酚类化合物和植物挥发物）的橡树（Q。在北莱茵-威斯特伐利亚（德国西部）的一个研究区域中，通过微阵列分析已经鉴定出的T和S橡树表型中的10种（粗壮）树。这种方法将使我们对Q的功能基因组学有更深入的了解。与食草昆虫的进食有关的强壮的。通过对栎属“耐高温”分子和生化标记的鉴定，为森林可持续经营提供决策支持。