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Dissertation Research: Fine Scale Evolutionary Adaptation to Temperature: A Comparative Approach Using Porcelain Crabs(genus Petrolisthes)

论文研究：对温度的精细尺度进化适应：使用瓷蟹（Petrolisthes）的比较方法

基本信息

批准号：
9700701
负责人：
George Somero
金额：
$ 0.95万
依托单位：
Stanford University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1997
资助国家：
美国
起止时间：
1997-06-15 至 1999-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9700701&HistoricalAwards=false
关键词：
Dissertation Research Fine Scale Evolutionary

项目摘要

Somero 9700701 Marine porcelain crabs of the genus Petrolisthes occur over a wide geographical range--from extremely hot tropical environments to cold north- and south temperate regions--and at widely different heights in the intertidal and subtidal zones. Thus, congeners of Petrolisthes encounter substantial differences in mean temperature and temperature range, making these crabs an excellent study system for examining evolutionary adaptation to temperature within a relatively narrow taxonomic group. The PI's initial studies of two congeners, P. eriomerus and P. cinctipes from low- and high intertidal zones, respectively, indicate pervasive adaptations to temperature and emersion stress. The proposed studies build on this work, and include, for several additional congeners: (1) determination of upper lethal temperature limits; (2) examination of the temperature sensitivity of structural and kinetic properties of lactate dehydrogenase; (3) determination of the function of leg membrane structures in gas exchange; and (4) development of a molecular phylogeny, using the sequence of the large subunit ribosomal RNA gene The latter experiments will enable analysis of the interspecific differences in physiological-biochemical-molecular traits to be analyzed in a phylogenetic context, thus enabling putative "adaptive" differences to be distinguished from changes not due to evolutionary responses to temperatures. .

Somero 9700701石油属海洋瓷蟹分布在广泛的地理范围内--从极端炎热的热带环境到寒冷的南北温带地区--而且在潮间带和潮下带的高度差别很大。因此，石龙蟹的同系物在平均温度和温度范围上遇到了很大的差异，使这些螃蟹成为在相对狭窄的分类学群体中检验对温度的进化适应的极好的研究系统。PI对分别来自低潮间带和高潮间带的两个同系物P.erimer us和P.cinctips的初步研究表明，它们普遍适应温度和重现压力。拟议的研究以这项工作为基础，并包括对其他几个同系物的研究：(1)确定致死温度上限；(2)检查乳酸脱氢酶结构和动力学性质的温度敏感性；(3)确定腿膜结构在气体交换中的功能；(4)利用大亚单位核糖体RNA基因序列发展分子系统学。后面的实验将能够在系统发育的背景下分析生理-生化-分子特性的种间差异，从而能够将假定的“适应性”差异与不是由于温度的进化反应而引起的变化区分开来。。