The role of RNases in mRNA and rRNA processing in the human pathogen Staphylococcus aureus

RNases 在人类病原体金黄色葡萄球菌 mRNA 和 rRNA 加工中的作用

• 批准号: 112927002
• 负责人: Professorin Dr. Christiane Wolz
• 金额: --
• 依托单位: Institut für Medizinische Mikrobiologie und Hygiene
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/112927002?language=en
• 关键词: role; RNases; mRNA; rRNA; processing

Bacteria are able to cope with dramatic environmental changes by rapidly altering gene expression. The maturation and degradation of RNA molecules are essential features in this regulation. The involved ribonucleases were mainly elucidated in the model organism Escherichia coli but in gram-positives the pathway of RNA degradation seems to be fundamentally different. We aim to discern RNA processing in the human pathogen Staphylococcus aureus using two model targets. First, we will focus on the global virulence regulator sae for which we could show that endonucleolytic cleavage leads to the origination of the major, very stable transcript. Especially we want to solve the questions which RNase is responsible for sae processing, what structural elements are necessary for cleavage, what influences the extraordinary transcript stability and what impact has processing on target gene expression? Since essentially the same enzymes are responsible for maturation of rRNA as for mRNA decay as a second model processing of the 16S rRNA will be studied. Global effects of RNases on the mRNA pool will be determined by microarray analysis. Our project will advance the understanding about the contribution of the RNA metabolism to the virulence potential of pathogenic bacteria.

细菌能够通过快速改变基因表达来科普剧烈的环境变化。RNA分子的成熟和降解是这种调节的基本特征。所涉及的核糖核酸酶主要在模式生物大肠杆菌中阐明，但在革兰氏阳性菌中，RNA降解途径似乎是根本不同的。我们的目标是辨别RNA加工在人类病原体金黄色葡萄球菌使用两个模型的目标。首先，我们将集中在全球毒力调节sae，我们可以表明，内切核酸裂解导致的主要，非常稳定的转录本的起源。特别是，我们想解决的问题是，RNA酶是负责sae加工，什么结构元件是必要的切割，是什么影响了非凡的转录稳定性和加工对靶基因的表达有什么影响？由于基本上相同的酶负责成熟的rRNA作为mRNA衰变的第二个模型的16S rRNA的加工将进行研究。RNA酶对mRNA库的总体影响将通过微阵列分析来确定。我们的项目将促进对RNA代谢对病原菌毒力潜力的贡献的理解。