The role of RNases in mRNA and rRNA processing in the human pathogen Staphylococcus aureus

细菌能够通过快速改变基因表达来应对剧烈的环境变化。RNA分子的成熟和降解是这一调控的基本特征。所涉及的核糖核酸酶主要在模式生物大肠杆菌中被阐明，但在革兰氏阳性中，RNA降解的途径似乎根本不同。我们的目的是辨别RNA加工在人类病原体金黄色葡萄球菌使用两个模型目标。首先，我们将重点关注全球毒力调节因子，我们可以证明核内溶分裂导致主要的，非常稳定的转录物的起源。特别是我们想要解决哪些RNase负责sae加工，哪些结构元件是切割所必需的，是什么影响了非凡的转录物稳定性以及加工对靶基因表达的影响。由于负责rRNA成熟的酶与负责mRNA衰变的酶本质上是相同的，因此我们将研究16S rRNA的第二种加工模式。rna酶对mRNA库的整体影响将通过微阵列分析确定。我们的项目将促进对RNA代谢对致病菌毒力潜力的贡献的理解。

Bacteria are able to cope with dramatic environmental changes by rapidly altering gene expression. The maturation and degradation of RNA molecules are essential features in this regulation. The involved ribonucleases were mainly elucidated in the model organism Escherichia coli but in gram-positives the pathway of RNA degradation seems to be fundamentally different. We aim to discern RNA processing in the human pathogen Staphylococcus aureus using two model targets. First, we will focus on the global virulence regulator sae for which we could show that endonucleolytic cleavage leads to the origination of the major, very stable transcript. Especially we want to solve the questions which RNase is responsible for sae processing, what structural elements are necessary for cleavage, what influences the extraordinary transcript stability and what impact has processing on target gene expression? Since essentially the same enzymes are responsible for maturation of rRNA as for mRNA decay as a second model processing of the 16S rRNA will be studied. Global effects of RNases on the mRNA pool will be determined by microarray analysis. Our project will advance the understanding about the contribution of the RNA metabolism to the virulence potential of pathogenic bacteria.