Structural biology of plant potyvirus and cytomegalovirus effector proteins (A03)

植物马铃薯Y病毒和巨细胞病毒效应蛋白的结构生物学（A03）

• 批准号: 113683888
• 金额: --
• 依托单位: Lehrstuhl für Biotechnik
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/113683888?language=en
• 关键词: Structural; biology; plant; potyvirus; cytomegalovirus

The structural determinants of plant potyvirus and metazoan cytomegalovirus (CMV) infection mechanisms will be characterized in A3. Higher oligomeric complexes formed by the CMV proteins IE1 and pUL50-pUL53 as well as plant potyvirus capsid proteins will be produced and crystallized. These crystallographic studies will be paralleled by fragment screening by X-ray crystallography efforts with available crystals of IE1 and pUL50-pUL53, crosslinking experiments and mutagenesis inquiries as well as in-depth studies by our collaborators from projects A2, A7, B3, C2 and C3.

植物马铃薯Y病毒和后生巨细胞病毒（CMV）感染机制的结构决定因素将在A3中描述。将产生并结晶由CMV蛋白IE1和pUL50-pUL53以及植物马铃薯Y病毒衣壳蛋白形成的高级寡聚复合物。这些晶体学研究将与使用 IE1 和 pUL50-pUL53 可用晶体的 X 射线晶体学工作进行碎片筛选、交联实验和诱变调查以及我们项目 A2、A7、B3、C2 和 C3 合作者的深入研究并行进行。