Administrative Supplement for Structural Dynamics in Biology Resource Year 2
生物资源第二年结构动力学行政补充
基本信息
- 批准号:10833964
- 负责人:
- 金额:$ 18.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-04-01 至 2026-03-31
- 项目状态:未结题
- 来源:
- 关键词:3D PrintAdministrative SupplementAutomobile DrivingBehaviorBiologicalBiological ProcessBiologyCardiovascular DiseasesCeramicsCharacteristicsCommunitiesComplementCryopreservationCrystallographyData CollectionDecision MakingDevelopmentDevicesDrug TargetingElectron Microscopy FacilityEnsureExposure toG-Protein-Coupled ReceptorsGeometryGoalsIntegral Membrane ProteinLaboratoriesLightLiquid substanceMalignant NeoplasmsMapsMeasurementMeasuresMental disordersMetalloproteinsMetalsMethodsMicrofluidicsOxidation-ReductionPhysiologic pulsePlant ResinsPositioning AttributePredispositionProcessPropertyPublishingRadiationRadiation induced damageResolutionResourcesRoentgen RaysSamplingSignal TransductionSolventsSourceSpeedStructureSynchrotronsSystemTechnologyTimeTrainingTranslatingViscosityWorkbasebiomaterial compatibilitydensitydetectordrug developmentexperimental studyfabricationmetalloenzymenovelopen sourceparent grantprogramsprototyperadiation mitigationstructural biologytherapeutic development
项目摘要
Project Summary – Administrative Supplement Request – 1P41GM139687-02 – Sebastien
Boutet (PI).
This administrative supplement request is driven by overall goals and aims of the Structural
Dynamics in Biology BTRR at SLAC National Accelerator Laboratory. The BTRR is aimed at
enhancing and developing the unique capabilities of the SLAC Linac Coherent Light Source
(LCLS) for biomedical applications. The requested supplement will support a broad user base
and further the goals of the DBPs and the TR&Ds.
By overcoming the limitations of radiation damage at the synchrotron, LCLS has been particularly
impactful in the study of large macromolecular machines that form small crystals with high solvent
content, making them delicate, difficult to cryo-preserve and extremely radiation sensitive. For
example, a major breakthrough was the application of SFX to examine crystals of intrinsic
membrane proteins, such as GPCRs, grown in LCP. GPCRs are the largest group of targets for
drug development, used to treat a wide variety of illnesses (e.g. cancer, cardiovascular disease,
and mental illness). LCLS is also impactful in the study of metalloenzymes, which are critical to
nearly all biological processes and as such represent a rich target space for therapeutics
development. High-resolution structural studies of metalloproteins are particularly challenging at
the synchrotron because the metal centers, especially those that are redox active, are very
susceptible to x-ray induced photoreduction. Further, the ultrafast (~40 fs) x-ray pulses produced
by the LCLS open new possibilities in directly observing dynamic processes involved in
macromolecular function. Moreover, many of the P41-derived developments that enable the rapid
collection of data using multiple small crystals, are applicable both at LCLS and at the synchrotron
to mitigate radiation damage. By expanding the capabilities at LCLS and at the SSRL synchrotron,
the BTRR opens more macromolecular machines to structural characterization, including time-
resolved studies over a wide range of biomedically relevant time scales. Integrating with, and
enhancing the existing programs at SSRL and LCLS, the BTRR will provide support, expertise
and training to the broad biomedical community.
This administrative supplement will enhance and expand the BTRR capabilities that are provided
to general users and to the P41 driving biomedical projects. The acquisition of a high speed x-ray
chopper will fill a critical need of the BTRR for efficient use of SSRL BL12-1 with small crystals
delivered by liquid/crystal injectors and for time-resolved measurements. In addition to achieving
microsecond time resolution, by breaking up the continuous x-ray beam into short pulses, the
chopper ensures crystals delivered by injectors are not destroyed by x-ray damage before they
are fully translated into the x-ray beam position, enabling exposure to unattenuated
monochromatic or pink-beam at BL12-1. In addition, this supplement requests a biocompatible
resin and ceramic 3D printer with 2-micron resolution that will allow for rapid prototyping and
optimization of sample injectors that will provide reliability and ease that will broaden the user
community. Finally, the proposal also requests a system to measure the conductivity of samples
in an automated manner which will allow to further the goals of the BTRR in characterizing sample
conditions and mapping those onto the ideal sample delivery method to be used for a given
sample, providing users with structured decision-making on how to best perform their experiment.
This will complement ongoing efforts in physicochemical characterization by adding conductivity,
an important parameter for electrokinetic sample delivery, to lookup tables to be published.
项目摘要-行政补充请求- 1P41GM139687-02 - Sebastien
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Sebastien Boutet其他文献
Sebastien Boutet的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Sebastien Boutet', 18)}}的其他基金
相似海外基金
A Longitudinal Qualitative Study of Fentanyl-Stimulant Polysubstance Use Among People Experiencing Homelessness (Administrative supplement)
无家可归者使用芬太尼兴奋剂多物质的纵向定性研究(行政补充)
- 批准号:
10841820 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
Proton-secreting epithelial cells as key modulators of epididymal mucosal immunity - Administrative Supplement
质子分泌上皮细胞作为附睾粘膜免疫的关键调节剂 - 行政补充
- 批准号:
10833895 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
Administrative Supplement: Life-Space and Activity Digital Markers for Detection of Cognitive Decline in Community-Dwelling Older Adults: The RAMS Study
行政补充:用于检测社区老年人认知衰退的生活空间和活动数字标记:RAMS 研究
- 批准号:
10844667 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
StrokeNet Administrative Supplement for the Funding Extension
StrokeNet 资助延期行政补充文件
- 批准号:
10850135 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
2023 NINDS Landis Mentorship Award - Administrative Supplement to NS121106 Control of Axon Initial Segment in Epilepsy
2023 年 NINDS 兰迪斯指导奖 - NS121106 癫痫轴突初始段控制的行政补充
- 批准号:
10896844 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
Biomarkers of Disease in Alcoholic Hepatitis Administrative Supplement
酒精性肝炎行政补充剂中疾病的生物标志物
- 批准号:
10840220 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
Administrative Supplement: Improving Inference of Genetic Architecture and Selection with African Genomes
行政补充:利用非洲基因组改进遗传结构的推断和选择
- 批准号:
10891050 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
Power-Up Study Administrative Supplement to Promote Diversity
促进多元化的 Power-Up 研究行政补充
- 批准号:
10711717 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
Administrative Supplement for Peer-Delivered and Technology-Assisted Integrated Illness Management and Recovery
同行交付和技术辅助的综合疾病管理和康复的行政补充
- 批准号:
10811292 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:
Sedentary behavior, physical activity, and 24-hour behavior in pregnancy and offspring health: the Pregnancy 24/7 Offspring Study Administrative Supplement
久坐行为、体力活动和 24 小时行为对怀孕和后代健康的影响:怀孕 24/7 后代研究行政补充
- 批准号:
10893074 - 财政年份:2023
- 资助金额:
$ 18.9万 - 项目类别:














{{item.name}}会员




