FT-Electron Paramagnetic Resonance Spectrometer

为升级电子顺磁共振光谱仪的部门共享设施提供配套支持，以允许进行时间域和多维傅立叶变换实验。所选择的光谱仪系统是Bruker ESP-380E-FT-300/FT/CW EPR光谱仪和DICE/随机脉冲Endor附件。该仪器将是普林斯顿大学校园内的第一个此类仪器，并将激发目前在普林斯顿无法获得的新的研究应用。首批12名研究人员将分布在三个学术部门和一个工业实验室，利用该仪器扩展他们在下列主要领域的研究计划：(1)多核金属酶的结构和功能，(2)细胞色素P450机制的分子袍，(3)锌指蛋白和血红素蛋白折叠，(4)电子顺磁共振探测肌动蛋白的构象变化，(5)观察和利用溶液ESR中的分子间多量子相干，(6)依赖硒的钼蝶呤酶。

Matching support for upgrading of a departmentally shared facility for Electron Paramagnetic Resonance Spectrometery to allow time-domain and multidimenional Fourier-transform experimentsis requested. The selected spectrometer system is the Bruker ESP-380E-FT-300/FT/CW EPR spectrometer and DICE/stochastic-Pulsed-ENDOR accesory. This instrument will be the first of its kind on the Princeton campus and will stimulate new research applications that are currently not accessible here. An initial group of 12 investigators distributed across three academic departments and an industrial laboratory will employ the instrument to extend their research programs in the following primary areas:(1) Structure and function of multi- nuclear metallo-enzymes, (2)molecular robes of the mechanism of Cytochrome P450, (3) Zinc finger proteins and heme protein folding, (4) Probing conformational changes in actin by EPR, (5) Observing and exploiting intermolecular multiple- quantum coherences in solution ESR, (6) Selenium- dependent molybdo-pterin enzymes.