FT-Electron Paramagnetic Resonance Spectrometer

傅里叶变换电子顺磁共振波谱仪

• 批准号: 9704238
• 负责人: Gerard Dismukes
• 金额: $ 16.88万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9704238&HistoricalAwards=false
• 关键词: FT; Electron; Paramagnetic; Resonance; Spectrometer

Matching support for upgrading of a departmentally shared facility for Electron Paramagnetic Resonance Spectrometery to allow time-domain and multidimenional Fourier-transform experimentsis requested. The selected spectrometer system is the Bruker ESP-380E-FT-300/FT/CW EPR spectrometer and DICE/stochastic-Pulsed-ENDOR accesory. This instrument will be the first of its kind on the Princeton campus and will stimulate new research applications that are currently not accessible here. An initial group of 12 investigators distributed across three academic departments and an industrial laboratory will employ the instrument to extend their research programs in the following primary areas:(1) Structure and function of multi- nuclear metallo-enzymes, (2)molecular robes of the mechanism of Cytochrome P450, (3) Zinc finger proteins and heme protein folding, (4) Probing conformational changes in actin by EPR, (5) Observing and exploiting intermolecular multiple- quantum coherences in solution ESR, (6) Selenium- dependent molybdo-pterin enzymes.

要求提供配套支助，以更新一个部门共用的电子顺磁共振光谱仪设施，以便进行时域和多维傅里叶变换实验。 所选用的光谱仪系统是Bruker ESP-380 E-FT-300/FT/CW EPR光谱仪和DICE/随机脉冲ENDOR附件。该仪器将是普林斯顿大学校园中的第一台此类仪器，并将刺激目前无法在这里获得的新的研究应用。 最初的一组12名研究人员分布在三个学术部门和一个工业实验室，将使用该仪器扩展他们在以下主要领域的研究计划：（1）多核金属酶的结构和功能，（2）细胞色素P450机制的分子袍，（3）锌指蛋白和血红素蛋白折叠，（4）用EPR探测肌动蛋白的构象变化，（5）观察和利用溶液ESR中的分子间多量子相干，（6）硒依赖的蝶呤酶。