Effects of Cyclic Strain and Oxidative Damage on Airway Epithelial Cell Permeability

循环应变和氧化损伤对气道上皮细胞通透性的影响

• 批准号: 9705600
• 负责人: Christopher Waters
• 金额: $ 18.82万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 1999-09-14
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9705600&HistoricalAwards=false
• 关键词: Effects; Cyclic; Strain; Oxidative; Damage

9705600 Waters Cells lining the airways of the lung protect the lung from inhaled toxins and harsh environmental conditions. Maintenance of this protective barrier depends upon the contacts between the cells and the re-growth of cells following injury. The cell barrier can be injured by oxidative species due to ozone, cigarette smoke, or infectious agents. The response of the cells to such injury and the repair of disruptions in the barrier are not well understood. Furthermore, although air flow in the lungs causes variations in the expansion of the airways, little is known about the effect of such mechanical strain on airway function. This proposal will investigate the maintenance and repair of the airway cell barrier following oxidant injury. A major goal of the proposed work is to develop techniques for the evaluation of barrier function while cells are mechanically stretched. There is currently no method for measuring the barrier properties of stretched cells. A novel device will be used to apply stretch to lung cells grown on elastic membranes. The barrier properties of the cells will be determined by measuring the electrical resistance across the cells. Using this tissue model, the response of the cells to oxidative damage will be measured, and the repair of the tissue will be evaluated. In addition, factors which may be important in protecting the cells or in enhancing repair will be investigated. This will lead to a better understanding of the injury and repair of the lung. ***

沃茨细胞排列在肺的气道中，保护肺免受吸入毒素和恶劣环境条件的影响。 这种保护屏障的维持取决于细胞之间的接触和损伤后细胞的再生长。 细胞屏障可被臭氧、香烟烟雾或传染性病原体引起的氧化物质损伤。 细胞对这种损伤的反应和屏障破坏的修复尚不清楚。 此外，尽管肺中的气流引起气道扩张的变化，但关于这种机械应变对气道功能的影响知之甚少。 本研究将探讨氧化损伤后气道细胞屏障的维持和修复。 拟议的工作的一个主要目标是开发技术的屏障功能的评价，而细胞是机械拉伸。 目前还没有测量拉伸电池的阻隔性能的方法。 一种新的装置将被用来对生长在弹性膜上的肺细胞施加拉伸。 电池的阻隔性能将通过测量电池两端的电阻来确定。 使用该组织模型，将测量细胞对氧化损伤的反应，并评估组织的修复。 此外，还将研究在保护细胞或增强修复方面可能重要的因素。 这将有助于更好地了解肺的损伤和修复。 ***