Effects of Cyclic Strain and Oxidative Damage on Airway Epithelial Cell Permeability

9705600水细胞排列在肺部的气道保护肺部免受吸入的毒素和恶劣的环境条件。这种保护屏障的维持取决于细胞之间的接触和损伤后细胞的再生。由于臭氧、香烟烟雾或传染性病原体，细胞屏障可被氧化物质损伤。细胞对这种损伤的反应和屏障破坏的修复尚不清楚。此外，尽管肺部的气流会引起气道扩张的变化，但人们对这种机械应变对气道功能的影响知之甚少。本研究将探讨氧化损伤后气道细胞屏障的维持和修复。提出的工作的一个主要目标是开发技术，以评估屏障功能，而细胞是机械拉伸。目前还没有测量拉伸细胞屏障特性的方法。一种新的装置将被用来对生长在弹性膜上的肺细胞施加拉伸。电池的阻隔特性将通过测量电池间的电阻来确定。使用该组织模型，将测量细胞对氧化损伤的反应，并评估组织的修复。此外，可能在保护细胞或促进修复中起重要作用的因素将被研究。这将有助于更好地了解肺的损伤和修复。＊＊＊

9705600 Waters Cells lining the airways of the lung protect the lung from inhaled toxins and harsh environmental conditions. Maintenance of this protective barrier depends upon the contacts between the cells and the re-growth of cells following injury. The cell barrier can be injured by oxidative species due to ozone, cigarette smoke, or infectious agents. The response of the cells to such injury and the repair of disruptions in the barrier are not well understood. Furthermore, although air flow in the lungs causes variations in the expansion of the airways, little is known about the effect of such mechanical strain on airway function. This proposal will investigate the maintenance and repair of the airway cell barrier following oxidant injury. A major goal of the proposed work is to develop techniques for the evaluation of barrier function while cells are mechanically stretched. There is currently no method for measuring the barrier properties of stretched cells. A novel device will be used to apply stretch to lung cells grown on elastic membranes. The barrier properties of the cells will be determined by measuring the electrical resistance across the cells. Using this tissue model, the response of the cells to oxidative damage will be measured, and the repair of the tissue will be evaluated. In addition, factors which may be important in protecting the cells or in enhancing repair will be investigated. This will lead to a better understanding of the injury and repair of the lung. ***