Modeling and evaluation of interactions between magnetic complexes and magnetic fields with MRI, M(H) and MRX methods - design of magnetic systems for targeting of magnetic complexes

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The aim of the project is to improve the understanding of particle magnetofection behavior and thereby to investigate the behavior of magnetic particles forming complexes with biological matrices (cells, viruses, bubbles). To achieve this, we will develop measurement techniques for determining the magnetic properties of nanoparticle complexes, e.g. of magnetically loaded cells, viruses or bubbles. Especially the changes in the hydrodynamic behavior of the nanoparticle complexes by incorporation in the biological matrix will be addressed. We will enhance and extend our particle movement simulation techniques towards speed, convergence and contributing forces. The detailed magnetic properties of nanoparticles and their complexes, which will be determined experimentally, will be incorporated into these simulations. Our simulations will be validated by phantom experiments using MRI and SQUID based magnetic measurement techniques. Additionally, we will calculate and design magnetic field sources for a variety of experiments and predict particle accumulations for different types of MNPs and magnetic field configurations in collaboration with the other projects. Finally, we will develop and validate magnetic resonance imaging methods to localize magnetic particles or magnetically labeled cells in vivo in small animals. We will develop forward models for calculating MR relaxation times of biological tissue containing magnetic particles on the basis of the nanoparticle properties measured by M(H), MPS and MRX. These models will be used to quantify particle concentrations in vivo with MRI.