CAA: Site Directed Mutagenesis of the Photosynthetic Water Oxidizing Complex

CAA：光合水氧化复合物的定点诱变

• 批准号: 9707280
• 负责人: Bridgette Barry
• 金额: $ 6万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9707280&HistoricalAwards=false
• 关键词: CAA; Site; Directed; Mutagenesis; Photosynthetic

9707280 Barpy This is a Career Advancement Award for Women Scientists and Engineers. Dr. Barry has been studying the structure and function of the photosynthetic water oxidizing complex (photosystem II). She has used a variety of techniques to achieve her research goals, including EPR and infrared spectroscopies. She has developed procedures to purify photosystem II from plants and cyanobacteria. In addition, she has been studying site directed mutants, generated in photosystem II from a cyanobacterium that can be grown photoheterotrophically. Dr. Barry will take a sabbatical leave in order to master the techniques of molecular biology and genetics, and will subsequently implement these techniques in her own laboratory. Photosystem II is present in plants, green algae, and prokaryotic cyanobacteria. The complex oxidizes water to oxygen. An understanding of the mechanism by which this occurs requires a combination of techniques, providing information about the structures of the redox active tyrosines in photosystem II, as well as information about the structural changes that occur at the manganese containing active site. Site directed mutagenesis can be used to assign spectral features to a particular residue in the enzyme. Ultimately, this combination of techniques provides new insight into the structure and function of photosystem II. ***

9707280芭比这是为女科学家和女工程师颁发的职业进步奖。巴里博士一直在研究光合作用水氧化复合体(光系统II)的结构和功能。她使用了各种技术来实现她的研究目标，包括EPR和红外光谱。她开发了从植物和蓝藻中提纯光系统II的程序。此外，她一直在研究定点突变，这种突变是在光系统II中从一种可以光异养生长的蓝藻中产生的。巴里博士将休假，以便掌握分子生物学和遗传学技术，随后将在自己的实验室实施这些技术。光系统II存在于植物、绿藻和原核蓝藻中。这种复合体将水氧化成氧气。要理解这种现象发生的机制，需要多种技术的结合，提供有关光系统II中氧化还原活性酪氨酸的结构信息，以及关于含锰活性部位发生的结构变化的信息。定点突变可用于将光谱特征分配给酶中的特定残基。最终，这种技术的结合为光系统II的结构和功能提供了新的见解。