CAA: Use of Transgenic Plants and Pollen to Study Pollen Extensins

贝丁格9707384 本实验室的长期研究目标是了解高等植物生殖过程中的分子事件，包括配子发生和授粉。花粉特异性基因产物的研究，包括Pex（花粉伸展蛋白）蛋白，一直是一个重大的努力的焦点。虽然我们已经对这些蛋白质进行了分子和定位研究，强烈表明它们在授粉中起着重要作用，但我们缺乏遗传数据来确定Pex蛋白质对花粉功能至关重要。获得该数据的一种方法是通过使用反义表达转基因植物。我们已经克隆了玉米和番茄版本的Pex基因。该提案将资助参观两个实验室，以了解如何转化番茄（和番茄花粉）和玉米，并分析所产生的转基因植物。我们的花粉功能分析包括突变基因型的遗传传递、花粉结构、花粉管萌发和结构以及花粉-雌蕊相互作用。

Bedinger 9707384 The long term objective of the research in my laboratory is to understand molecular events in the reproduction of higher plants, including gametogenesis and pollination. The study of pollen-specific gene products, including the Pex (Pollen-extensin) proteins, has been the focus of a major effort. Although we have performed molecular and localization studies on these proteins that strongly suggest that they play an important role in pollination, we lack the genetic data that firmly establishes that the Pex proteins are essential for pollen function. One way to obtain this data is through the use of antisense-expressing transgenic plants. We have cloned both maize and tomato versions of the Pex genes. This proposal is to fund visits to two laboratories to learn how to transform tomato (and tomato pollen) and maize, and to analyze the transgenic plants that are produced. Our assays for pollen function encompass genetic transmission of mutant genotypes, pollen structure, pollen tube germination and structure, and pollen-pistil interactions.