Glycosylation of Recombinant Proteins Research Planning Grant

9711130 Harcum这项研究计划拨款提案涉及生物反应器条件对蛋白质糖基化模式的影响，这似乎是一个具有商业意义和科学研究的领域。该研究计划将研究在连续培养（化学）中生长的哺乳动物细胞，并确定诸如搅拌、pH值、温度、生长速率等变量的影响。电泳和色谱分离加上质谱和核磁共振分析将用于结构测定。＊＊＊

9711130 Harcum This Research Planning Grant proposal addresses what appears to be a commercially significant and scientifically under- investigated area, the effect of bioreactor conditions on the glycosylation patterns of protein. The research program would study mammalian cells grown in continuous culture (chemosts) and determine the effect of variables such as agitation, pH, temperature, growth rates, etc. Electrophoretic and chromatographic separations coupled with mass spec and NMR analyses will be used in structural determinations. ***