SGER: Inclusion body formation in E. coli: What triggers this outcome?

化学、生物工程、环境和运输系统SGER：在大肠杆菌中形成包涵体：是什么触发了这一结果？CBET 0738162当前应用的目的是表征重组蛋白与宿主细胞之间在包涵体(IB)形成方面的相互作用，并控制和/或缓解这一现象。假设IB的形成是一个细胞介导的过程，由重组蛋白和热休克反应的组成部分之间的相互作用引起。拟议的高风险研究将获得初步数据，以支持关于重组大肠杆菌中包涵体形成的新假说。

Chemical, Bioengineering, Environmental and Transport SystemsSGER:Inclusion Body Formation in E. coli: What Triggers This Outcome?Sarah W. HarcumClemson UniversityProposal No. CBET 0738162The objective of the current application is to characterize interactions between recombinant proteins and the host cell, with respect to inclusion body (IB) formation and to control and/or alleviate this phenomenon. The hypothesis is that IB formation is a cell-mediated process caused by interactions between the recombinant protein and components of the heat-shock response. The proposed high-risk research will obtain preliminary data to support a novel hypothesis on inclusion body formation in recombinant E. coli.