SGER: Inclusion body formation in E. coli: What triggers this outcome?

化学、生物工程、环境和运输系统SGER：大肠杆菌中包涵体的形成。大肠杆菌：是什么触发了这种结果？莎拉·W HarcumClemson大学提案号CBET 0738162本申请的目的是表征重组蛋白和宿主细胞之间关于包涵体（IB）形成的相互作用，并控制和/或减轻这种现象。假设IB形成是由重组蛋白和热休克反应组分之间的相互作用引起的细胞介导的过程。 该高风险研究将获得初步数据，以支持重组大肠杆菌中包涵体形成的新假设。杆菌

Chemical, Bioengineering, Environmental and Transport SystemsSGER:Inclusion Body Formation in E. coli: What Triggers This Outcome?Sarah W. HarcumClemson UniversityProposal No. CBET 0738162The objective of the current application is to characterize interactions between recombinant proteins and the host cell, with respect to inclusion body (IB) formation and to control and/or alleviate this phenomenon. The hypothesis is that IB formation is a cell-mediated process caused by interactions between the recombinant protein and components of the heat-shock response. The proposed high-risk research will obtain preliminary data to support a novel hypothesis on inclusion body formation in recombinant E. coli.