CAREER: Stress Response in Recombinant Eschericia coli: A Research and Educational Tool

9732957 Harcum该职业计划的目标是增强对重组大肠杆菌应激反应的了解，并整合生物技术研究和化学工程师教育。 本提案的研究部分将使用实验来增加对重组蛋白生产过程中蛋白水解过程的细胞控制的基本理解。 将监测蛋白酶活性水平，并测定切割敏感性肽键。 将开发和完善一个模型，以改善重组发酵的控制。 该信息将具有降低发酵和下游纯化成本的双重益处。 该计划的教育部分侧重于教育生物技术原理的化学工程师，以满足对这一专业知识日益增长的需求。 重组E.大肠杆菌将成为本科生和研究生研究与教育一体化的模型系统。 ***

9732957 Harcum The objective of this career program is to enhance the understanding of the stress response in recombinant Escherichia coli and to integrate biotechnology research and education of chemical engineers. The research portion of this proposal will use experiments to increase the basic understanding of cellular control of proteolytic processes during recombinant protein production. Protease activity levels will be monitored as well as the cleavage sensitive peptide bonds determined. A model will be developed and refined to improve the control of recombinant fermentations. This information will have the dual benefit of reducing both fermentation and downstream purification costs. The education portion of the program is focused towards educating chemical engineers in biotechnology principles to meet the growing demand for this expertise. The stress response in recombinant E. coli will be model system for the integration of research and education for undergraduate and graduate students. ***