Soluble Complexes of DNA with Cationic Block Copolymers

DNA 与阳离子嵌段共聚物的可溶性复合物

• 批准号: 9712657
• 负责人: Alexander Kabanov
• 金额: $ 20万
• 依托单位: University of Nebraska Medical Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9712657&HistoricalAwards=false
• 关键词: Soluble; Complexes; DNA; Cationic; Block

9712657 Kabanov This proposal will investigate complexes formed between nucleic acids and cationic block copolymers and their potential use in delivering DNA into cells. Both water soluble complexes (using polyethylene oxide/polyspermine copolymers) and complexes soluble in non-polar media (polystyrene/poly N-ethyl vinylpyridinium bromide) will be synthesized and investigated. Extensive characterization of the complexes is planned via electron microscopy, circular dichroism, light scattering, etc. The rate of DNA release from the complex under several "cellular" mechanisms will also be studied. ***

该提案将研究在核酸和阳离子嵌段共聚物之间形成的复合物及其在将DNA输送到细胞中的潜在用途。水溶性配合物（使用聚乙烯氧化物/聚精胺共聚物）和可溶于非极性介质（聚苯乙烯/聚n-乙基乙烯基溴化吡啶）的配合物将被合成和研究。广泛的表征配合物是计划通过电子显微镜，圆二色性，光散射等。在几种“细胞”机制下，DNA从复合物中释放的速率也将被研究。＊＊＊