Soluble Complexes of DNA with Cationic Block Copolymers

9712657 Kabanov该提案将研究核酸和阳离子嵌段共聚物之间形成的复合物及其在将DNA递送到细胞中的潜在用途。 水溶性复合物（使用聚环氧乙烷/聚精胺共聚物）和复合物可溶于非极性介质（聚苯乙烯/聚N-乙基乙烯基溴化吡啶）将被合成和研究。 计划通过电子显微镜，圆二色性，光散射等的复合物的广泛表征。也将研究从复合物的DNA释放速率下的几个“细胞”机制。 ***

9712657 Kabanov This proposal will investigate complexes formed between nucleic acids and cationic block copolymers and their potential use in delivering DNA into cells. Both water soluble complexes (using polyethylene oxide/polyspermine copolymers) and complexes soluble in non-polar media (polystyrene/poly N-ethyl vinylpyridinium bromide) will be synthesized and investigated. Extensive characterization of the complexes is planned via electron microscopy, circular dichroism, light scattering, etc. The rate of DNA release from the complex under several "cellular" mechanisms will also be studied. ***