Soluble Complexes of DNA and Cationic Polymers for Gene Delivery

该项目的目标是开发可溶的DNA与阳离子聚合物的络合物，用于将基因输送到细胞。该提案描述的目标有三个：(1)检测不同分子结构的DNA/聚阳离子复合体的基因转移效率；(2)研究将复合体输送到细胞内的细胞机制；(3)评价含有附着蛋白和多肽的DNA/聚阳离子复合体的基因转移效率。

The objective of this project is to develop soluble complexes of DNA with cationic polymers for use in gene delivery to cells. The objectives described in the proposal are threefold: (1) to examine the efficacy of gene transfer of DNA/polycation complexes of various molecular architectures; (2) to study cellular mechanisms for delivery of the complexes into the cell; and (3) to evaluate the efficacy of gene transfer of DNA/polycation complexes containing attached proteins and peptides.