CAREER: Structural Studies of Transcription Regulation by RNA-Protein Interactions

9722392 Palmer描述了一个职业发展计划，该计划将核磁共振波谱的结构/功能研究和教学相结合。该研究项目将为理解rna -蛋白复合物的分子识别以及小羔羊样噬菌体的转录终止/反终止提供分子基础。最初的目标是表征HK022 Nun蛋白与Box B RNA序列之间的相互作用。本建议的教育目标是开发高分辨率核磁共振波谱学的教学和自学资源。高分辨率核磁共振波谱是学术和工业环境中化学和生物研究中越来越重要的组成部分，越来越多的大学开设了核磁共振波谱课程。教育工作是基于教科书，题为蛋白质核磁共振光谱学：原理和实践，共同撰写了该提案的PI。课程材料将用于哥伦比亚大学教授的“生物大分子的核磁共振波谱学”课程，并将通过互联网发布。了解蛋白质特异性识别和与其他分子相互作用的机制是化学、生物物理学和生物技术领域的一个关键研究问题。本提案的研究部分将使用核磁共振波谱来阐明蛋白质和RNA分子之间相互作用的本质，这对分子识别很重要。教育部分将开发以计算机为基础的材料，用于高等本科和研究生阶段的核磁共振波谱学教学。教育材料将补充教材，题为蛋白质核磁共振光谱学：原理和实践，共同撰写的PI。* * *

9722392 Palmer A career development program is described that integrates research and pedagogy of structure/function by NMR spectroscopy. The research program will provide a molecular basis for understanding molecular recognition in RNA-protein complexes as well as transcription termination/antitermination in lambdoid coliphages. The initial goal is the characterization of the interaction between HK022 Nun protein and Box B RNA sequences. The educational objectives of this proposal are the development of resources for the teaching and self-study of high resolution NMR spectroscopy. High-resolution NMR spectroscopy is an increasingly important component of chemical and biological research in academic and industrial settings, and courses in NMR spectroscopy are offered at an expanding number of universities. The educational efforts are based on the textbook, entitled Protein NMR Spectroscopy: Principles and Practice, co-authored by the PI of this proposal. Course materials will be used in the course "NMR spectroscopy of biological macromolecules", taught at Columbia University, and will be distributed via the Internet. Understanding the mechanisms by which proteins specifically recognize and interact with other molecules is a critical research problem in chemistry, biophysics, and biotechnology. The research component of this proposal will use NMR spectroscopy to elucidate the nature of the interactions between proteins and RNA molecules that are important for molecular recognition. The educational component will develop computer-based materials for teaching NMR spectroscopy at the advanced undergraduate and graduate university levels. The educational materials will complement the textbook, entitled Protein NMR Spectroscopy: Principles and Practice, co-authored by the PI. ***