Reporter Genes to Study Seed Germination and Dormancy

研究种子萌发和休眠的报告基因

• 批准号: 9722978
• 负责人: Kent Bradford
• 金额: $ 34.3万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9722978&HistoricalAwards=false
• 关键词: Reporter; Genes; Study; Seed; Germination

9722978 Bradford Seed germination is a critical developmental transition in the plant life cycle. While seeds are generally quite tolerant of environmental stresses, seedlings are highly vulnerable to extremes of temperature or water availability. Considering that our understanding of the molecular events that lead to germination is scant, the work proposed here is designed to provide molecular markers for seed germination. One obstacle to a better understanding is that seeds, even from a genetically uniform background, germinate at different times. The work will study the mechanisms that lead to this heterogeneity using the expression of GFP reporters under the control of promoters for germination-specific genes which are available. Not only will the pattern of expression be monitored, the reporter protein will be used to select stages of germination. Additionally, the influence of plant growth regulators on the activity of these promoters in seeds with different degree of dormancy or germination will be studied. The application of results from this work for the agronomically important trait germination is obvious.

9722978 Bradford种子萌发是植物生命周期中的一个关键发育转变。虽然种子通常对环境胁迫具有相当的耐受性，但幼苗非常容易受到极端温度或水的影响。考虑到我们对导致萌发的分子事件的了解很少，这里提出的工作旨在为种子萌发提供分子标记。更好地理解的一个障碍是，即使来自遗传上相同的背景，种子也会在不同的时间发芽。这项工作将研究导致这种异质性的机制，使用绿色荧光蛋白报告基因的启动子控制下的发芽特异性基因，这是可用的。不仅将监测表达模式，报告蛋白将用于选择发芽阶段。此外，还将研究植物生长调节剂对不同休眠或萌发程度种子中这些启动子活性的影响。 这项工作的结果应用于农艺学上重要的性状发芽是显而易见的。