Nuclear Regulation of Mitochondrial Genome Integrity

线粒体基因组完整性的核调控

• 批准号: 9728329
• 负责人: Sally Mackenzie
• 金额: $ 15.62万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 1999-07-07
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728329&HistoricalAwards=false
• 关键词: Nuclear; Regulation; Mitochondrial; Genome; Integrity

9728329 Mackenzie The plant mitochondrial genome is unusually variable in its size and highly complex in its organization when compared to its counterparts in yeast and animal systems. Of particular interest for this project is the observation that distinct mitochondrial DNA molecules, each containing only a portion of the genetic information, are retained in the genome at strikingly different relative copy number. What accounts for this unusual organization in plants is not well understood. It is clear that many features of the mitochondrial genome, including DNA copy number, are under nuclear gene control. This project is designed to test the hypothesis that nuclear-directed copy number control of mitochondrial DNA molecules serves as a means of suppressing mitochondrial mutations in plants. To test this hypothesis, the CHM gene in Arabidopsis thaliana has been cloned. Mutations in this gene result in the amplification of mitochondrial DNA molecules that contain mutations. Thus, characterization of this gene, its product, and its mode of action should allow a direct test of the stated hypothesis. We plan to confirm the identity of the CHM clone, to localize its gene product, and to genetically test its function.

与酵母和动物系统中的线粒体基因组相比，植物线粒体基因组在大小上异常多变，在组织结构上高度复杂。这个项目特别感兴趣的是观察到不同的线粒体DNA分子，每个分子只包含一部分遗传信息，以截然不同的相对拷贝数保留在基因组中。是什么导致了植物中这种不寻常的组织尚不清楚。很明显，线粒体基因组的许多特征，包括DNA拷贝数，都受到核基因的控制。该项目旨在验证线粒体DNA分子的核定向拷贝数控制作为抑制植物线粒体突变的一种手段的假设。为了验证这一假设，拟南芥的CHM基因已经被克隆。这个基因的突变会导致包含突变的线粒体DNA分子的扩增。因此，对该基因、其产物及其作用方式的表征应该允许对所述假设进行直接测试。我们计划确认CHM克隆的身份，定位其基因产物，并对其功能进行基因测试。