Conjugation of Indole-3-acetic Acid to Proteins and Peptides

3-吲哚乙酸与蛋白质和肽的缀合

• 批准号: 9723999
• 负责人: Todd Cooke
• 金额: $ 30.5万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9723999&HistoricalAwards=false
• 关键词: Conjugation; Indole; acetic; Acid; Proteins

9723999 Cohen Indole-3-acetic acid is found in plants in free and conjugated forms. One of the less well studied forms of conjugated IAA are a class of peptides/proteins that are modified by the amide linkage of IAA to the protein. The work will address several problems of this class of IAA conjugates. Occurrence of the conjugates will be studied during fruit and seed development in particular, and in other organs of the plant during vegetative development. IAA peptides will be isolated and immunological and peptide mapping studies will be conducted. Transcripts for proteins from which these peptides derive will be obtained, based on the mapping data and these transcripts will be used for the analysis of expression of the genes during development. The studies are expected to provide an understanding of the, possibly multiple, roles of this class of modified proteins in relationship to growth regulator biology.

9723999科恩吲哚-3-乙酸以游离态和结合态存在于植物中。研究较少的一种结合IAA的形式是一类通过IAA与蛋白质的酰胺键修饰的多肽/蛋白质。这项工作将解决这类IAA共轭的几个问题。特别是在果实和种子发育期间，以及营养发育期间植物的其他器官中，将研究这种偶联物的出现情况。将分离IAA多肽，并进行免疫学和多肽图谱研究。根据图谱数据，将获得这些多肽来源的蛋白质的转录本，这些转录本将用于分析这些基因在发育过程中的表达。这些研究有望提供对这类修饰蛋白与生长调节剂生物学关系的可能多重作用的理解。