Conjugation of Indole-3-Acetic Acid to Proteins and Peptides

3-吲哚乙酸与蛋白质和肽的缀合

• 批准号: 0111530
• 负责人: Gary Gardner
• 金额: $ 33万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0111530&HistoricalAwards=false
• 关键词: Conjugation; Indole; Acetic; Acid; Proteins

Indole-3-acetic acid is found in plants in both free and conjugated form. One of the less well-studied forms of conjugated IAA are a unique class of proteins where IAA is a prosthetic group attached directly to the protein structure. An increasing body of evidence suggests that regulation of protein stability is important to auxin function, thus making these modified proteins of particular interest. The first gene for a protein covalently modified by IAA was recently cloned from bean and found to be related to proteins present in several other plant species, including the model plant Arabadopsis. Four lines of study will further characterize these proteins in plants. 1) Experiments will define, on the molecular level, the expression, regulation and function of the bean 35 kDa protein. 2) Previous studies which focused on bean will be extended, starting with cloning the respective genes, to related proteins from species more amenable to genetic manipulation, such as Arabadopsis and Medicago.3) IAA-protein gene expression will be analyzed at the tissue and subcellular level using reporter genes and immunological techniques.4) The attachment of IAA to the proteins will be analyzed using complex liquid chromatography mass spectrometry methods that will allow rapid identification of this class of modified proteins. These studies should provide the biochemical, tissue and molecular information necessary to further understand the possible roles of this unique class of modified proteins in relation to phytohormone regulation.

3-吲哚乙酸以游离形式和结合形式存在于植物中。 共轭 IAA 的研究较少，是一类独特的蛋白质，其中 IAA 是直接附着在蛋白质结构上的辅基。 越来越多的证据表明，蛋白质稳定性的调节对于生长素功能很重要，因此使这些修饰的蛋白质特别令人感兴趣。 最近从豆类中克隆了第一个经 IAA 共价修饰的蛋白质基因，并发现该基因与其他几种植物物种（包括模式植物拟南芥）中存在的蛋白质相关。四项研究将进一步表征植物中的这些蛋白质。 1) 实验将在分子水平上定义豆子 35 kDa 蛋白的表达、调节和功能。 2) 先前以豆类为重点的研究将从克隆各自的基因开始，扩展到更适合基因操作的物种中的相关蛋白质，例如拟南芥和苜蓿。3) 将使用报告基因和免疫学技术在组织和亚细胞水平上分析 IAA 蛋白基因表达。4) 将使用复杂的液相色谱分析 IAA 与蛋白质的附着情况 质谱方法将允许快速鉴定此类修饰蛋白质。这些研究应该提供必要的生化、组织和分子信息，以进一步了解这一类独特的修饰蛋白在植物激素调节方面的可能作用。