Molecular Genetics of the Drosophila Myosuppressin Gene

果蝇肌抑制素基因的分子遗传学

• 批准号: 9724141
• 负责人: Ruthann Nichols
• 金额: $ 26.37万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9724141&HistoricalAwards=false
• 关键词: Molecular; Genetics; Drosophila; Myosuppressin; Gene

This project focuses on understanding how peptides in the central nervous system regulate various physiological functions. In particular, the PI is interested in a peptide that inhibits heart rate. This peptide is referred to as DMS or dromyosuppressin. The DMS peptide is encoded by the dromyosuppressin gene. DMS inhibits heart rate in a dose dependent manner when introduced into the animal. The PI is conducting research in an animal amenable to genetic analysis and proposes to generate mutations in the gene that encodes DMS to better understand the role of the peptide in animal physiology. After obtaining these mutations, the PI will use molecular and physiological techniques to determine when the peptide is expressed and how altering the presence of DMS might affect the animal. Because this peptide is conserved in structure and function, our work will have broad application to other organisms.

这个项目的重点是了解中枢神经系统中的多肽如何调节各种生理功能。特别是，PI对一种抑制心率的多肽感兴趣。这种多肽被称为DMS或Dromyosuppressin。DMS多肽由Dromyosuppressin基因编码。当将DMS引入动物体内时，DMS以剂量依赖的方式抑制心率。PI正在对一种可以进行遗传分析的动物进行研究，并提议在编码DMS的基因中产生突变，以更好地了解这种肽在动物生理学中的作用。在获得这些突变后，PI将使用分子和生理技术来确定多肽何时表达，以及改变DMS的存在可能如何影响动物。由于该多肽在结构和功能上是保守的，我们的工作将会在其他生物上有广泛的应用。