U.S.-Czech Cell Biology Research on Nuclear Myosin I

美国-捷克细胞核肌球蛋白 I 细胞生物学研究

• 批准号: 9724168
• 负责人: Primal de Lanerolle
• 金额: $ 3.97万
• 依托单位: University of Illinois at Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-15 至 2000-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9724168&HistoricalAwards=false
• 关键词: U.S.; Czech; Cell; Biology; Research

INT 9724168 de Lanerolle This U.S.-Czech research project between Primal de Lanerolle of the University of Illinois, Chicago, and Pavel Hozak of the Institute of Experimental Medicine, Prague, will attempt to identify a non-filamentous motor protein, myosin I, by cloning and sequencing the cDNA for it. Their intent is to initiate the process of defining the physiological functions of this protein by using immunogold electron microscopy to identify the structures it associates with in the nucleus. Results of the sequencing will be compared to published sequences for members of the myosin superfamily to determine whether the 120 kDa protein is myosin I, some other unconventional myosin, or a totally new actin, ATP and calmodulin binding ATPase in the nucleus. Central to the effort is an antibody to myosin I, which the U.S. and Czech researchers have produced and affinity purified from bovine adrenal glands. This antibody inhibits the actin-activated ATPase activity of purified adrenal myosin I but not that of purified adrenal myosin II. These antibodies recognize a 120 kDa protein that is larger than the antigen (16 kDa) in the cytoplasm and in the nucleus. Comparative studies using different antibodies to myosin I suggest that these antibodies recognize a unique, previously undescribed myosin I isoform that is an intranuclear actin-based molecular motor. If successful, this research effort to unequivocally identify the 120 kDa protein could make fundamental contributions to our understanding of the cellular functions of myosin and the biology of the cell nucleus. This project in cellular organization fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Central Europe to combine complementary talents and pool resources in areas of strong mutual interest and competence. ??

INT 9724168 de Lanerolle 这个美国-芝加哥伊利诺斯大学的Primal de Lanerolle和布拉格实验医学研究所的Pavel Hozak之间的捷克研究项目将试图鉴定一种非丝状运动蛋白，肌球蛋白I，他们的目的是通过使用免疫金电子显微镜来识别它相关的结构，从而开始定义这种蛋白质的生理功能的过程。在原子核中。 测序结果将与肌球蛋白超家族成员的公开序列进行比较，以确定120 kDa蛋白质是否是肌球蛋白I，一些其他非常规肌球蛋白，或一个全新的肌动蛋白，ATP和钙调蛋白结合ATP酶在细胞核中。 这项工作的核心是肌球蛋白I的抗体，美国和捷克的研究人员已经从牛肾上腺中生产并亲和纯化了这种抗体。 该抗体抑制纯化肾上腺肌球蛋白I的肌动蛋白激活ATP酶活性，但不抑制纯化肾上腺肌球蛋白II的活性。 这些抗体识别比细胞质和细胞核中的抗原（16 kDa）大的120 kDa蛋白质。 使用不同的肌球蛋白I抗体的比较研究表明，这些抗体识别一个独特的，以前未描述的肌球蛋白I亚型，是一个核内肌动蛋白为基础的分子马达。 如果成功的话，这项明确鉴定120 kDa蛋白质的研究工作将为我们理解肌球蛋白的细胞功能和细胞核的生物学做出根本性的贡献。 这个细胞组织项目实现了促进科学知识的计划目标，使美国和中欧的领先专家能够在具有强烈共同利益和能力的领域联合收割机互补人才和资源。 ??