Acquisition and Operation of a Light Microscopy and Digital Imaging Facility

光学显微镜和数字成像设备的购置和操作

• 批准号: 9724473
• 负责人: Timothy Karr
• 金额: $ 59.01万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9724473&HistoricalAwards=false
• 关键词: Acquisition; Operation; Light; Microscopy; Digital

Karr 9724473 This proposal describes a shared confocal microscopy facility at the University of Chicago that will open new lines of research for a broad range of scientists. To meet the immediate and anticipated demands of the user base, the proposed facility will include two stand-alone systems, a Zeiss LSM confocal systems have complementary strengths, but both are capable of producing high-resolution confocal images and are suitable for either fixed or living specimens. Visualization of datasets will be accomplished using Macintosh PowerPC and Silicon Graphics computers and associated software for image analysis and display. Because biological imaging and visualization is still very much a "hands on" enterprise and remains for the most part quite technical, the proposed facility is designed to provide a "user friendly" environment to made the technology accessible to individual investigators, their trainees and students. To this end, the facility will be a staffed by an FTE whose mission will be to train and assist researchers who wish to utilize the capabilities of these powerful machines.

Karr 9724473 该提案描述了芝加哥大学的一个共享共聚焦显微镜设施，该设施将为广泛的科学家开辟新的研究领域。 为了满足用户群的直接和预期需求，拟议的设施将包括两个独立的系统，一个是蔡司LSM系统，另一个是LSM系统，两者具有互补的优势，但都能够产生高分辨率的共焦图像，适用于固定或活体标本。 将使用Macintosh PowerPC和Silicon Graphics计算机及相关图像分析和显示软件实现数据集的可视化。 由于生物成像和可视化在很大程度上仍然是一个“动手”的企业，在很大程度上仍然是相当技术性的，拟议的设施旨在提供一个“用户友好”的环境，使技术提供给个人调查人员，他们的受训人员和学生。 为此，该设施将配备一名全职员工，其使命是培训和协助希望利用这些强大机器能力的研究人员。