Nuclear Migration and Early Zygotic Gene Action in Drosophila

果蝇的核迁移和早期合子基因作用

• 批准号: 8802152
• 负责人: Timothy Karr
• 金额: $ 22.16万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-15 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8802152&HistoricalAwards=false
• 关键词: Nuclear; Migration; Early; Zygotic; Gene

The specific goals of this proposal are: 1) To achieve, using newly developed immunocytochemical techniques, a detailed picture of both nuclear and cytoskeletal organizations present in Drosophila embryos during the cleavage stages of development. 2) To critically analyze the exact details of how the cytoskeletal elements tubulin and actin reorganize as the cleavage nuclei enter the periphery. These results will be augmented with real- time observations of living embryos viewed with Nomarski DIC optics and documented with video technology. These results, in conjunction with the previous work will provide the descriptive foundations for which many known Drosophila mutations affecting early development can be analyzed. 3) To examine in greater detail the effects mutations in the engrailed and even-skipped genes have on early development. Mutations in these two genes are lethal to the embryo, and disrupt segmentation in a reproducible and spatially specific manner. This proposal is designed to further our understanding of the relationship between these genes and cytoskeletal elements in the embryo that provide structure and movement, with special emphasis on the actin and tubulin-based cytoskeletons. In the long term, an understanding of how these and other genes act to build the body plan of Drosophila should tell us how similar genes work in higher eukaryotes including humans. %%% Fertilization of an egg by the sperm initiates a developmental program that results in a fully viable adult animal. This developmental program is completely specified by the contents of the fertilized egg, and the challenge facing developmental biologists is to decipher exactly how the information that is present in the egg is first stored, and then subsequently utilized and processed as the embryo develops. Dr. Karr's work will provide key information about the very earliest events following fertilization.

本提案的具体目标是：1）实现， 使用新开发的免疫细胞化学技术， 细胞核和细胞骨架组织的图片， 果蝇胚胎在发育的卵裂阶段。 （二） 批判性地分析细胞骨架 微管蛋白和肌动蛋白分子重组为卵裂核 进入外围。 这些结果将用真实的- 使用Nomarski DIC观察活胚胎的时间观察 光学和视频技术记录。 这些结果，在 结合以前的工作将提供描述性的 许多已知的果蝇突变影响了 可以分析早期的发展。 3)以更大的 详细介绍了影响突变的enrailed和甚至跳过 基因对早期发育的影响 这两个基因的突变 对胚胎是致命的，并破坏胚胎的分裂。 可重复的和空间特定的方式。 这项建议是 旨在加深我们对 胚胎中的这些基因和细胞骨架元素 结构和运动，特别强调肌动蛋白和 基于微管蛋白的细胞骨架。 从长远来看， 这些基因和其他基因是如何作用于 果蝇应该告诉我们相似的基因如何在高等生物中起作用。 包括人类在内的真核生物 %%% 精子使卵子受精， 一个能使一个完全存活的成年人 动物 这一发展计划完全由 受精卵的内容物，以及面临的挑战 发育生物学家的任务是破译 首先存储鸡蛋中存在的信息，然后 随后在胚胎发育时使用和加工。博士 卡尔的工作将提供关于最早的 受精后的事件。