Ecophysiology of Heat-Mediation Abatement of Cytoplasmic Incompatibility

[604287 .卡尔这项拟议的研究调查了一种细菌感染对它所感染的宿主繁殖成功的影响。因为细菌影响繁殖，它深刻地影响了宿主的种群和进化生物学。沃尔巴克氏体细菌降低或消除了雄性宿主与未感染的雌性繁殖后代的能力。这一建议的初步研究表明，在野外遇到的轻微热休克可以减少生殖不相容。研究将：(1)确定拟沙蚕幼虫自然经历热休克的频率；(2)确定可以治愈不相容性的温度范围和发育阶段；(3)区分ci的减少是通过直接杀死/抑制沃尔巴克氏体和/或通过热休克蛋白的表达增加来介导的；(4)实验研究自然热休克是否真的能治愈自然种群的生殖不亲和性。拟议的研究有望整合生态和进化生理学、生殖生物学、分子生物学、种群生物学和细菌学的各个方面，并可能产生对这些领域都有意义的结果。最终，这些信息可能有助于合理设计新的生物虫害防治形式。

9604287 Karr The proposed research investigates the effect of a bacterial infection on reproductive success of the host it infects. Because the bacteria influences reproduction, it profoundly impacts both the population and evolutionary biology of its host. The bacterium, Wolbachia, reduces or abolishes the ability of male hosts to father offspring with uninfected females. Research preliminary to this proposal establishes that mild heat shock such as that encountered in the wild can reduce the reproductive incompatibility. The research would: (1) Determine the frequency with which D. simulans larvae naturally experience heat shock; (2) Determine the range of temperatures and the developmental stages at which the incompatibility can be cured; (3) Distinguish whether CI-abatement occurs via outright killing/suppression of Wolbachia and/or is mediated by increased expression of heat-shock proteins; (4) Experimentally examine whether natural heat shock actually cures the reproductive incompatibility in natural populations. The proposed research promises to integrate diverse aspects of ecological and evolutionary physiology, reproductive biology, molecular biology, population biology, and bacteriology, and is likely to yield results significant to each of these areas. Ultimately, this information could be useful for the rational design of new forms of biological pest control.