U.S.-Japan Cooperative Science: Novel Characterization of Humic Substances and Their Role in the Behavior of Environmental Toxic Substances Using HPLC/MS/MS

美日合作科学：使用 HPLC/MS/MS 对腐殖质及其在环境有毒物质行为中的作用进行新表征

• 批准号: 9726617
• 负责人: Roger Minear
• 金额: $ 3.7万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9726617&HistoricalAwards=false
• 关键词: U.S.; Japan; Cooperative; Science; Novel

9726617 Minear This award supports a three year collaborative research project between Professor Roger Minear of the University of Illinois, Urbana-Champaign and Professor Saburo Matsui of Kyoto University in Japan. The researchers will undertake a study of novel characterization of humic substances and their role in the behavior of environmental toxic substances. The underlying hypothesis of the overall research effort is that natural organic matter, and humic substances as a subset, affect the environmental toxicology of anthropogenic compounds through complex interactions that can affect membrane transport and mechanisms of toxicological reactions. Following separation by membrane fractionation and employing size exclusion columns and other partitioning principles, samples will be analyzed using high performance liquid chromatography (HPLC) interfaced with tandem mass spectrometry (MS/MS). This work should advance knowledge about humic substances and their combination with selected environmental contaminants. It is anticipated that characterization of molecular weight and fragmentation patterns will be obtained at a level leading to information regarding actual molecular weights not previously available. The goal is to develop new processes to enhance the identification of the specific molecular structure of humic materials. This project brings together the efforts of two laboratories that have complementary expertise, research capabilities and equipment. The University of Illinois brings expertise in the isolation and concentration of natural organic matter and humic substances plus new approaches to the cooperative research endeavor. The Japanese researchers have an ongoing research effort on the study of micropollutant effects in Lake Biwa, the largest fresh water body in Japan that isof great economic importance to several major cities including Osaka and Kyoto. Recent acquisition of major equipment, along with the state-of the-art analytic al capabilities of researchers at Kyoto University, will offer opportunities for advanced studies of drinking water research and advanced toxicological techniques. Through the exchange of ideas and technology, this project will broaden our base of basic knowledge and promote international understanding and cooperation. ***

小行星9726617 该奖项支持伊利诺伊大学香槟分校的Roger Minear教授和日本京都大学的Saburo Matsui教授之间为期三年的合作研究项目。 研究人员将对腐殖物质的新特性及其在环境有毒物质行为中的作用进行研究。 整个研究工作的基本假设是，天然有机物和腐殖物质作为一个子集，通过复杂的相互作用，可以影响膜运输和毒理学反应机制，影响人为化合物的环境毒理学。 在通过膜分离并采用分子排阻色谱柱和其他分配原理进行分离后，将使用高效液相色谱法（HPLC）与串联质谱法（MS/MS）接口分析样品。这项工作应该推进有关腐殖物质及其与选定的环境污染物的组合的知识。 预计将获得分子量和断裂模式的表征，从而获得先前无法获得的实际分子量信息。 其目标是开发新的工艺，以提高腐殖物质的特定分子结构的识别。 该项目汇集了两个实验室的努力，这两个实验室具有互补的专业知识、研究能力和设备。 伊利诺伊大学在天然有机物和腐殖物质的分离和浓缩方面拥有专业知识，并为合作研究奋进提供了新的方法。 日本研究人员正在对日本最大的淡水湖-琵琶湖的微污染物影响进行研究，该湖对大坂和京都等几个主要城市具有重要的经济意义。 最近获得的主要设备，沿着的国家最先进的分析能力的研究人员在京都大学，将提供机会，为饮用水研究和先进的毒理学技术的先进研究。 通过交流思想和技术，该项目将扩大我们的基础知识，促进国际理解与合作。 ***