Consequences of Nuclear Domain 10 Elimination and Shielding

核域 10 消除和屏蔽的后果

• 批准号: 9728398
• 负责人: Gerd Maul
• 金额: $ 24万
• 依托单位: Wistar Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728398&HistoricalAwards=false
• 关键词: Consequences; Nuclear; Domain; 10; Elimination

9728398 Maul The nucleus is a highly compartmentalized structure in which there is selective trafficking of proteins and RNA across the nuclear envelope, as well as targetting of these macromolecules to specific sites within the nucleus. Although there does not seem to be a need for trafficking of DNA, which in the case of the cell's DNA is a permanent resident of the nucleus, there now is evidence that the DNA of at least some viruses is targeted to specific domains within the nucleus. The significance of this is not understood. It is reasonably assumed, however, that the targeting is important in viral transcription and replication. These studies will examine in detail the function of nuclear targeting of viral DNA to specific domains, termed ND10, that have been identified in preliminary studies. The long term goal is to define the molecular basis of this DNA targeting and its general significance for nuclear structure, gene targeting, and viral infection. ***

细胞核是一个高度区隔化的结构，其中蛋白质和RNA在核膜上有选择性地运输，以及这些大分子靶向到细胞核内的特定位置。虽然似乎不需要贩运DNA，就细胞的DNA是细胞核的永久居民而言，现在有证据表明，至少一些病毒的DNA是针对细胞核内的特定区域的。这一点的意义还没有被理解。然而，可以合理地假设，靶向在病毒转录和复制中是重要的。这些研究将详细检查病毒DNA核靶向特定结构域的功能，称为ND10，已在初步研究中确定。长期目标是确定这种DNA靶向的分子基础及其在核结构、基因靶向和病毒感染方面的一般意义。＊＊＊