Acquisition of a Confocal Laser Scanning Microscope

9202417毛尔这项提案要求为多用户组使用的共焦显微镜提供资金。主要用户将在项目中长期使用该仪器。项目包括对分化过程中不断变化的染色体结构域以及特定和新发现的核结构域进行三维重建，在发育或维甲酸诱导分化或皮肤重建中通过共培养改变细胞的相互关系，在整个发育中胚胎中不同的mRNA分布，以及特定基因在完整核中的相对定位(定位)。除了这些较大的项目外，维斯塔尔研究所的不同实验室还确定并启动了较小的项目，特别是正在开发的结构生物学方案。这些工作将由首席调查员监督。项目多种多样，需要一种有效的方法来研究0.1到1米范围内的细胞或组织结构，样本的厚度为0.1-1毫米。共焦显微镜的先进状态为我们提供了实现目标的手段。

9202417 Maul This proposal requests the funds for a confocal microscope to be used by a multi-user group. The major users will utilize the instrument in projects over the long term. Projects include the 3D reconstruction of changing chromosomal domains during differentiation and of specific and newly found nuclear domains, the changing inter- relationships of cells during development or induced differentiation by retinoic acids or by coculture in skin reconstruction, the different distribution of mRNA in whole developing embryos, and the relative localization of specific genes in the intact nucleus (mapping). Aside from these larger projects minor projects have been identified and initiated by different laboratories at The Wistar Institute in particular in the developing structural biology program. These will be supervised by the principal investigator. Projects are diverse and need an efficient way to study cell or tissue architecture in the 0.1 to 1 m range in specimens that have a thickness of 0.1-1-mm. Confocal microscopy in its advanced state provides us with the means to achieve our goals.