Acquisition of a Confocal Laser Scanning Microscope

购买共焦激光扫描显微镜

• 批准号: 9202417
• 负责人: Gerd Maul
• 金额: $ 12.71万
• 依托单位: Wistar Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9202417&HistoricalAwards=false
• 关键词: Acquisition; Confocal; Laser; Scanning; Microscope

9202417 Maul This proposal requests the funds for a confocal microscope to be used by a multi-user group. The major users will utilize the instrument in projects over the long term. Projects include the 3D reconstruction of changing chromosomal domains during differentiation and of specific and newly found nuclear domains, the changing inter- relationships of cells during development or induced differentiation by retinoic acids or by coculture in skin reconstruction, the different distribution of mRNA in whole developing embryos, and the relative localization of specific genes in the intact nucleus (mapping). Aside from these larger projects minor projects have been identified and initiated by different laboratories at The Wistar Institute in particular in the developing structural biology program. These will be supervised by the principal investigator. Projects are diverse and need an efficient way to study cell or tissue architecture in the 0.1 to 1 m range in specimens that have a thickness of 0.1-1-mm. Confocal microscopy in its advanced state provides us with the means to achieve our goals.

9202417 Maul该提案要求为多用户组使用的共焦显微镜提供资金。 主要用户将在项目中长期使用该工具。 项目包括在分化过程中改变染色体结构域和特定的和新发现的核结构域的3D重建，在发育过程中或通过视黄酸或通过皮肤重建中的共培养诱导分化的细胞的变化的相互关系，mRNA在整个发育胚胎中的不同分布，以及完整细胞核中特定基因的相对定位（映射）。 除了这些大型项目外，Wistar研究所的不同实验室还确定并启动了一些小型项目，特别是在发展结构生物学计划方面。 这些将由主要研究者监督。 项目是多样化的，需要一种有效的方法来研究厚度为0.1 -1 mm的标本中0.1 - 1 m范围内的细胞或组织结构。先进的共聚焦显微镜为我们提供了实现目标的手段。