Translational Control of RuBPCase Gene Expression
RuBPase 基因表达的翻译控制
基本信息
- 批准号:9728547
- 负责人:
- 金额:$ 27.58万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-03-01 至 2002-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
9728547 Berry/Gollnick Amaranth is a dicotyledonous grain plant that uses the highly efficient c4 pathway of photosynthesis. In amaranth, the expression of genes encoding the large subunit (LSU, produced from the plastid-encoded rbcL gene) and small subunit (SSU, produced from nuclear-encoded rbcS genes) of ribulose 1,5-bisphosphate (RuBPCase) is strongly regulated by light and by developmental processes. Previously, it has been shown that very rapid changes in synthesis of the LSU and SSU polypeptides are induced in response to changes in illumination, and that these changes are due to regulation at the translations/level. In addition, alterations in translation from rbcL and RbcS mRNAs are associated with changes in leaf photosynthetic capacity, and appear to occur during various stages of leaf development. The research to be conducted will analyze light-dependent mRNA/protein interactions that occur at the 5' UTR of rbcL mRNA. The light-dependent binding of two 47 kDa proteins and the formation of a slow-migrating gel mobility shift complex to processed rbcL 5'UTR sequences are tightly associated with our observations of light-induced activation of RuBPCase LSU translation. To better understand the role of mRNA/protein interactions in regulating the synthesis of this plastid-encoded photosynthetic polypeptide, the predominant 47 kDa rbcL 5' UTR mRNA binding proteins will be characterized and their associated factors identified. The formation and composition of the slow-migrating rbcL 5' UTR RNA binding complex produced by plastid extracts from light-grown plants will be investigated, as will the fast-migrating complex produced by the dark-plastid extracts. In addition, it will be determined how processing differences in the length of the rbcL 5' UTR prevent or enhance light-dependent protein binding and complex formation. In the long term, the information acquired from these in depth biochemical studies will be used to characterize the kinetic properties of the light-dependent protein/R NA interactions, and to determine the role of the RNA binding proteins using a transgenic C4 dicot system. These investigations will provide novel insights into mechanisms that regulate photosynthetic gene expression at the translational level in the C4 dicot amaranth and other higher plants. In higher plants light serves both as a primary energy source and as an environmental signal that regulates development. Investigations into light-regulated responses in plants are of fundamental importance in determining how factors controlling plant development respond to environmental stimuli. The process of protein translation is an integral part of the overall pathway of gene expression in all organisms. Rapid changes in the translation of specific mRNAs may be a mechanism for adapting to sudden environmental changes that could affect the survival of the organism. In addition to providing unique perspectives about the genetic control of photosynthesis, this basic research with amaranth will aid in its development as an agriculturally important crop for food production and for industry.
9728547 Berry/Gollnick阿马兰斯是一种双子叶谷物植物,利用高效的c4光合作用途径。在阿马兰斯中,编码1,5-二磷酸核酮糖(RuBPCase)大亚基(LSU,由质体编码的rbcL基因产生)和小亚基(SSU,由核编码的rbcS基因产生)的基因的表达受到光和发育过程的强烈调控。以前,已经表明LSU和SSU多肽合成的非常快速的变化是响应于光照的变化而诱导的,并且这些变化是由于翻译/水平的调节。此外,从rbcL和RbcS mRNA的翻译的改变与叶片光合能力的变化,并出现在叶片发育的各个阶段。即将进行的研究将分析在rbcL mRNA的5' UTR处发生的光依赖性mRNA/蛋白质相互作用。两个47 kDa蛋白质的光依赖性结合和缓慢迁移的凝胶迁移率变化复合物的形成与处理rbcL 5 'UTR序列紧密相关,与我们观察到的光诱导激活RuBPCase LSU翻译。为了更好地理解mRNA/蛋白质相互作用在调节这种质体编码的光合多肽的合成中的作用,将表征主要的47 kDa rbcL 5' UTR mRNA结合蛋白并鉴定其相关因子。将研究由来自光生长植物的质体提取物产生的缓慢迁移的rbcL 5' UTR RNA结合复合物的形成和组成,以及由暗质体提取物产生的快速迁移的复合物。此外,还将确定rbcL 5' UTR长度的加工差异如何阻止或增强光依赖性蛋白结合和复合物形成。从长远来看,从这些深入的生物化学研究中获得的信息将用于表征光依赖蛋白/RNA相互作用的动力学特性,并确定RNA结合蛋白的作用,使用转基因C4双子叶植物系统。这些研究将为C4双子叶植物阿马兰斯和其他高等植物在翻译水平上调控光合基因表达的机制提供新的见解。 在高等植物中,光既是主要的能量来源,又是调节发育的环境信号。研究植物的光调节反应对于确定控制植物发育的因素如何响应环境刺激具有重要意义。蛋白质翻译过程是所有生物体中基因表达整体途径的组成部分。特定mRNA翻译的快速变化可能是适应可能影响生物体生存的突然环境变化的机制。除了提供关于光合作用遗传控制的独特观点外,这项对阿马兰斯的基础研究将有助于其作为粮食生产和工业的重要农业作物的发展。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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James Berry其他文献
Biologically-engineered Pediatric Tri-tube Valved Conduit Evaluated 52 Weeks in the Growing Lamb
- DOI:
10.1080/24748706.2021.1901474 - 发表时间:
2021-06-01 - 期刊:
- 影响因子:
- 作者:
Zeeshan Syedain;Bee Haynie;Sandra Johnson;Mathew Lahti;James Berry;John Carney;Jirong Lee;Ryan Hill;Kirk Hansen;Greeshma Nair;Richard Bianco;Robert Tranquillo - 通讯作者:
Robert Tranquillo
Ophthalmological evaluation of facial burns in a regional burns centre
- DOI:
10.1016/j.burns.2019.10.025 - 发表时间:
2020-06-01 - 期刊:
- 影响因子:
- 作者:
James Berry;Jack Ashley;Steven Jeffery - 通讯作者:
Steven Jeffery
TCT-407 Transcatheter Pediatric Valve From Novel Biologic Regenerative Tissue
- DOI:
10.1016/j.jacc.2022.08.478 - 发表时间:
2022-09-20 - 期刊:
- 影响因子:
- 作者:
Zeeshan Syedain;Rick Murphy;James Berry;Allie Haarstad - 通讯作者:
Allie Haarstad
Practical requirements for the successful implementation and subsequent dissemination of the redefined kilogram
- DOI:
10.1016/j.vacuum.2015.07.007 - 发表时间:
2015-10-01 - 期刊:
- 影响因子:
- 作者:
Stuart Davidson;James Berry;Kilian Marti - 通讯作者:
Kilian Marti
Using digital imaging to create the ‘wow’ factor for patients
- DOI:
10.14219/jada.archive.2004.0417 - 发表时间:
2004-10-01 - 期刊:
- 影响因子:
- 作者:
James Berry - 通讯作者:
James Berry
James Berry的其他文献
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{{ truncateString('James Berry', 18)}}的其他基金
MRI: Acquisition of a Confocal Microscopy System for Research and Education
MRI:购买用于研究和教育的共焦显微镜系统
- 批准号:
0923133 - 财政年份:2009
- 资助金额:
$ 27.58万 - 项目类别:
Standard Grant
Post-transcriptional control of C4 RbcS Gene Expression in Flaveria bidentis
Flaveria bidentis 中 C4 RbcS 基因表达的转录后控制
- 批准号:
0544234 - 财政年份:2006
- 资助金额:
$ 27.58万 - 项目类别:
Continuing Grant
Post-transcriptional Control of rbcL Gene Expression in the C4 dicots Amaranth and Flaveria Bidentis
C4 双子叶植物苋菜和黄顶菊中 rbcL 基因表达的转录后控制
- 批准号:
0110411 - 财政年份:2001
- 资助金额:
$ 27.58万 - 项目类别:
Continuing Grant
U.S.-Australia Cooperative Research: Analysis of Transcriptional & Post-Transcription Gene Constructs in Transgenic C4 Plants
美国-澳大利亚合作研究:转录分析
- 批准号:
9724775 - 财政年份:1998
- 资助金额:
$ 27.58万 - 项目类别:
Standard Grant
Translational Control of RuBPCase Gene Expression in Amaranth
苋菜中 RuBPCase 基因表达的翻译控制
- 批准号:
9316806 - 财政年份:1994
- 资助金额:
$ 27.58万 - 项目类别:
Continuing Grant
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