Isothermal Titration Calorimetry for Biological Research

用于生物学研究的等温滴定量热法

• 批准号: 9729490
• 负责人: Ashutosh Chilkoti
• 金额: $ 4.85万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9729490&HistoricalAwards=false
• 关键词: Isothermal; Titration; Calorimetry; Biological; Research

Eight investigators from five departments of Duke University will purchase an isothermal titration calorimeter. This instrument will provide the investigators with access to a state-of-the-art isothermal calorimetry instrument which will allow elucidation of the fundamental thermodynamics of molecular recognition processes in research currently underway in the laboratories of the investigators. Among the systems to be studied are: (1) protein - ligands and their interactions; (2) signal peptides in lipid bilayers; (3) carbonic anhydrase and metal affinity; (4) extracellular matrix proteins and cell receptors; (5) membranes and macromolecular binding; (6) interactions of ATP and other agents with DNA topoisomerase; and (7) neurotrophins in cortical neurons. The new instrument, in conjunction with ongoing structural and biochemical studies will permit a molecular level dissection of structure-function relationships in these important systems.

来自杜克大学五个系的八名研究人员将购买一台等温滴定量热仪。 该仪器将为研究人员提供一种最先进的等温量热仪，可以阐明研究人员实验室目前正在进行的研究中分子识别过程的基本热力学。待研究的系统包括：（1）蛋白质-配体及其相互作用;（2）脂质双层中的信号肽;（3）碳酸酐酶和金属亲和力;（4）细胞外基质蛋白和细胞受体;（5）膜和大分子结合;（6）ATP和其他试剂与DNA拓扑异构酶的相互作用;以及（7）皮层神经元中的神经营养因子。新的仪器，结合正在进行的结构和生物化学研究，将允许在这些重要系统中的结构-功能关系的分子水平解剖。