Isothermal Titration Calorimetry for Biological Research

来自杜克大学五个系的八名研究人员将购买一台等温滴定量热仪。该仪器将为研究人员提供使用最先进的等温量热仪的途径，该仪器将允许阐明研究人员实验室目前正在进行的研究中的分子识别过程的基本热力学。需要研究的系统包括：(1)蛋白质-配体及其相互作用；(2)脂双层中的信号肽；(3)碳酸氢酶和金属亲和力；(4)细胞外基质蛋白和细胞受体；(5)膜和大分子结合；(6)ATP和其他药物与DNA拓扑异构酶的相互作用；(7)皮质神经元中的神经营养因子。新的仪器，结合正在进行的结构和生化研究，将允许在分子水平上剖析这些重要系统中的结构-功能关系。

Eight investigators from five departments of Duke University will purchase an isothermal titration calorimeter. This instrument will provide the investigators with access to a state-of-the-art isothermal calorimetry instrument which will allow elucidation of the fundamental thermodynamics of molecular recognition processes in research currently underway in the laboratories of the investigators. Among the systems to be studied are: (1) protein - ligands and their interactions; (2) signal peptides in lipid bilayers; (3) carbonic anhydrase and metal affinity; (4) extracellular matrix proteins and cell receptors; (5) membranes and macromolecular binding; (6) interactions of ATP and other agents with DNA topoisomerase; and (7) neurotrophins in cortical neurons. The new instrument, in conjunction with ongoing structural and biochemical studies will permit a molecular level dissection of structure-function relationships in these important systems.