Isothermal Titration Calorimetry for Biological Research

来自杜克大学5个系的8名研究人员将购买等温滴定量热计。该仪器将为研究人员提供最先进的等温量热仪，这将使研究人员能够阐明目前正在实验室进行的分子识别过程的基本热力学。待研究的系统包括：(1)蛋白质-配体及其相互作用；(2)脂质双分子层信号肽；(3)碳酸酐酶与金属亲合力；(4)细胞外基质蛋白和细胞受体；(5)膜与大分子结合；(6) ATP和其他试剂与DNA拓扑异构酶的相互作用；(7)皮层神经元中的神经营养因子。新仪器与正在进行的结构和生化研究相结合，将允许在分子水平上解剖这些重要系统的结构-功能关系。

Eight investigators from five departments of Duke University will purchase an isothermal titration calorimeter. This instrument will provide the investigators with access to a state-of-the-art isothermal calorimetry instrument which will allow elucidation of the fundamental thermodynamics of molecular recognition processes in research currently underway in the laboratories of the investigators. Among the systems to be studied are: (1) protein - ligands and their interactions; (2) signal peptides in lipid bilayers; (3) carbonic anhydrase and metal affinity; (4) extracellular matrix proteins and cell receptors; (5) membranes and macromolecular binding; (6) interactions of ATP and other agents with DNA topoisomerase; and (7) neurotrophins in cortical neurons. The new instrument, in conjunction with ongoing structural and biochemical studies will permit a molecular level dissection of structure-function relationships in these important systems.