CAREER: Smart Protein-Polymer Conjugates for Biotechnology

9733009 Chilkoti拟议研究的主要目标是通过连接到活性位点附近的蛋白质支架上的热触发分子开关可逆地调节蛋白质-配体亲和力。 该设计包括一种新的两步策略，结合遗传循环置换与酶辅助的反向蛋白质水解，以产生最终的蛋白质-聚合物缀合物。 PI设想未来开发的可再生的，半连续的生物传感器的基础上permustat-poly（NIPAAm）的结构，显示“解离速率”控制的亲和力调制。 研究活动是由一个教育计划，解决两个主要目标的补充：建立一个外展计划，高中学生工程的杜克学校内，和生物医学工程课程的扩展，包括在基因工程，分子生物学和蛋白质工程领域的新选修课程。 ***

9733009 Chilkoti The major goal of the proposed research is to reversibly modulate protein-ligand affinity by a thermally-triggered molecular switch attached to the protein scaffold in the vicinity of the active site. The design includes a novel two-step strategy, combining genetic circular permutation with enzyme-assisted reversed proteolysis, to produce the final protein-polymer conjugate. The PI envisions future development of a regenerable, semi-continuous biosensor based on permustat-poly (NIPAAm) constructs that display "off-rate" controlled affinity modulation. The research activity is complemented by an educational program which addresses two primary aims: the establishment of an outreach program for high school students within the Duke School of Engineering, and the expansion of the Biomedical Engineering curriculum to include new elective courses in the fields of genetic engineering, molecular biology and protein engineering. ***