RAPID: Combined Antigen and Serology Rapid Test for COVID-19

RAPID：针对 COVID-19 的抗原和血清学联合快速检测

• 批准号: 2029361
• 负责人: Ashutosh Chilkoti
• 金额: $ 11.93万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2021-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2029361&HistoricalAwards=false
• 关键词: RAPID; Combined; Antigen; Serology; Rapid

COVID-19 outbreak is currently a pandemic disease and public health emergency in many countries. Population-wide testing is critical for controlling the outbreak and periodic retesting will be important to prevent future spikes in transmission. The research team of Prof. Chilkoti at Duke University aims to develop a rapid and sensitive test that detects coronavirus (SARS-CoV-2) for infection diagnosis and patient-derived antibodies for immunity assessment. The benefit of this combined format is that if the test results are negative for SARS-CoV-2 on symptomatic patients, serology results will indicate if a previous infection existed. For asymptomatic patients, hidden active infections can also be detected. The sensing method is designed to enable sample handling and testing to be easily performed by personnel who have minimal medical training. This project on developing a simple to use and widely deployable biosensor provides research training opportunities to graduate students and helps to prepare a skilled workforce for the biosensing and biomedical engineering fields.The multiplex point-of-care test is designed for detecting the viral nucleocapsid protein (N) from nasal swabs and patient-derived antibodies against the viral S1 spike protein (S1) from a few drops of blood. It uses a single, standardized microfluidic chip that allows testing of both swab buffers and whole blood. Upon addition of a patient's samples, the assay automatically advances to completion without user intervention. It employs a highly sensitive and inexpensive handheld detector, the D4Scope, that does not require an external power source or laboratory infrastructure. The results can be wirelessly transmitted to a remote server.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

目前，新冠肺炎 (COVID-19) 疫情已成为许多国家的流行病和突发公共卫生事件。全人群检测对于控制疫情至关重要，定期重新检测对于防止未来传播高峰也很重要。 杜克大学 Chilkoti 教授的研究团队旨在开发一种快速、灵敏的测试，可检测冠状病毒（SARS-CoV-2）以进行感染诊断，并检测患者来源的抗体以进行免疫评估。 这种组合形式的好处是，如果有症状患者的 SARS-CoV-2 检测结果呈阴性，血清学结果将表明是否存在先前的感染。 对于无症状患者，也可以发现隐藏的活动性感染。该传感方法旨在使受过最少医疗培训的人员能够轻松地进行样品处理和测试。 该项目旨在开发一种简单易用且可广泛部署的生物传感器，为研究生提供研究培训机会，并有助于为生物传感和生物医学工程领域培养熟练的劳动力。多重即时护理测试旨在检测鼻拭子中的病毒核衣壳蛋白 (N) 和来自几滴血液中的针对病毒 S1 刺突蛋白 (S1) 的患者衍生抗体。 它使用单个标准化微流控芯片，可以测试拭子缓冲液和全血。 添加患者样本后，检测会自动完成，无需用户干预。 它采用高度灵敏且廉价的手持式探测器 D4Scope，不需要外部电源或实验室基础设施。 结果可以无线传输到远程服务器。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优点和更广泛的影响审查标准进行评估，被认为值得支持。