CAREER: Calbindin D28k: A Synthetic Peptide Approach

职业生涯：Calbindin D28k：一种合成肽方法

• 批准号: 9733174
• 负责人: Karin Akerfeldt
• 金额: $ 10.14万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-15 至 1998-12-04
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9733174&HistoricalAwards=false
• 关键词: CAREER; Calbindin; D28k; Synthetic; Peptide

The focus of this research is the elucidation of the calcium binding properties and domain organization of calbindin D28K, a protein abundant in specific nervous tissue and one of the larger EF-hand members, containing six putative EF-hands. The strategy involves the synthesis of each EF-hand as a 33-residue peptide sequence, which can then be characterized individually or in combinations with other EF-hands. Methodology for the for connecting the EF-hands to provide fragments comprising more than one EF-hand is also described. The focus of the educational activities involves the the education of students through laboratory experience and through course and curriculum development in organic chemistry. With this Faculty Early Development (CAREER) award, the Organic and Macromolecular Chemistry Program is supporting the research and educational activities of Dr. Karin S. Akerfeldt of the Department of Chemistry at Rutgers University, Camden. Professor Akerfeldt will focus her research efforts on protein structure-function relationship studies using a synthetic approach that will delineate calcium binding properties and domain organization of the EF-hand proteins. This class of proteins is present in eukaryotes and is involved in a vast number of physiological processes. Dr. Akerfeldt's educational activities are involved with course and curriculum development.

这项研究的重点是阐明钙结合蛋白 D28K 的钙结合特性和结构域组织，钙结合蛋白 D28K 是一种在特定神经组织中丰富的蛋白质，也是较大的 EF 手成员之一，包含六个假定的 EF 手。 该策略涉及将每个 EF-hand 合成为 33 个残基的肽序列，然后可以单独或与其他 EF-hand 组合进行表征。 还描述了用于连接EF手以提供包含多于一个EF手的片段的方法。 教育活动的重点包括通过实验室经验以及有机化学课程和课程开发对学生进行教育。 凭借这一教师早期发展（职业）奖，有机和高分子化学项目正在支持卡姆登罗格斯大学化学系的 Karin S. Akerfeldt 博士的研究和教育活动。 Akerfeldt 教授将把她的研究重点放在蛋白质结构-功能关系研究上，使用合成方法来描述 EF 手蛋白的钙结合特性和结构域组织。 这类蛋白质存在于真核生物中，并参与大量的生理过程。 阿克费尔特博士的教育活动涉及课程和课程开发。