CAREER: Calbindin D28k: A Synthetic Peptide Approach

本研究的重点是阐明钙结合 钙结合蛋白D28 K的性质和结构域组织， 在特定的神经组织和一个较大的EF-手成员中， 包含六个推定的EF手。 该战略涉及综合 每个EF-手作为33个残基的肽序列，然后可以将其 单独或与其他EF手组合表征。 用于连接EF手以提供片段的方法 包括多于一个EF指针的系统。 的重点 教育活动包括通过以下方式教育学生： 实验室经验，并通过课程和课程开发， 有机化学 有了这个教师早期发展（职业生涯）奖， 有机和高分子化学计划是支持 Karin S博士的研究和教育活动。阿克费尔特 罗格斯大学化学系，卡姆登。 教授 Akerfeldt将把她的研究重点放在蛋白质结构-功能上 使用综合方法进行关系研究， EF-手的钙结合性质和结构域组织 proteins. 这类蛋白质存在于真核生物中， 参与了大量的生理过程 Akerfeldt医生 教育活动涉及课程与课程 发展

The focus of this research is the elucidation of the calcium binding properties and domain organization of calbindin D28K, a protein abundant in specific nervous tissue and one of the larger EF-hand members, containing six putative EF-hands. The strategy involves the synthesis of each EF-hand as a 33-residue peptide sequence, which can then be characterized individually or in combinations with other EF-hands. Methodology for the for connecting the EF-hands to provide fragments comprising more than one EF-hand is also described. The focus of the educational activities involves the the education of students through laboratory experience and through course and curriculum development in organic chemistry. With this Faculty Early Development (CAREER) award, the Organic and Macromolecular Chemistry Program is supporting the research and educational activities of Dr. Karin S. Akerfeldt of the Department of Chemistry at Rutgers University, Camden. Professor Akerfeldt will focus her research efforts on protein structure-function relationship studies using a synthetic approach that will delineate calcium binding properties and domain organization of the EF-hand proteins. This class of proteins is present in eukaryotes and is involved in a vast number of physiological processes. Dr. Akerfeldt's educational activities are involved with course and curriculum development.