BINDING PROPERTIES OF HUMAN CENTRIN 2 AND CALBINDIN D28K

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 人中心蛋白2（20 kDa）和钙结合蛋白D28 K（30 kDa）是Ca 2+结合蛋白家族的成员。 我们使用PLIMSTEX，通过质谱，滴定和H/D交换研究蛋白质配体相互作用，以探索这些蛋白质的钙结合特性。 这种策略可以显示结合钙后的构象变化、亲和常数以及结合化学计量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human centrin 2 (20 kDa) and calbindin D28K (30 kDa) are members of the family of Ca2+-binding proteins. We use PLIMSTEX, the study of Protein Ligand Interactions by Mass Spectrometry, Titration, and H/D Exchange, to explore the calcium binding properties of these proteins. This strategy can show the conformational change upon binding calcium, the affinity constants, and also the binding stoichiometry.