CAREER: An Internet-Based Multimedia Approach to Teaching Developmental Neurobiology with Integrated Research on Neuronal Migration

职业：基于互联网的多媒体教学方法，结合神经元迁移的综合研究来教授发育神经生物学

• 批准号: 9734550
• 负责人: Patricia Phelps
• 金额: $ 44万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 2004-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9734550&HistoricalAwards=false
• 关键词: CAREER; Internet; Based; Multimedia; Approach

9734550 PHELPS Dr. Phelps' Early Career award contains both educational and research components related to the field of developmental neurobiology. The educational aim is to build an internet-based multimedia learning system for a new undergraduate course on developmental neurobiology, in collaboration with Dr. Gambhir and colleagues at the Crump Institute for Biological Imaging at UCLA. During nervous system development, many complex tissue movements occur that are difficult for students to understand using the typical 2-dimensional drawings from textbooks, yet they come alive when presented as 3-dimensional animations. Designing colorful animations depicting these complicated tissue movements is an important goal of this project. An additional focus is to incorporate data from scientists working in the field of developmental neurobiology. For example, time-lapse video microscopy demonstrating observations of living neurons will be used in classroom presentations to introduce students to exciting new research techniques. Students are able to review class presentations over the web, and utilize interactive quizzes as study aids. Such evaluation tools will help the students, but also will provide the teachers with information about the effectiveness of this new internet-based learning system. The aim of the research component is to determine what structures and/or molecules guide the unique migration of a group of chemically defined neurons during early spinal cord development. The neurons under study, called the "U-derived" cells, use acetylcholine as their neurotransmitter and can be identified by both immunological and histochemical methods. Between embryonic days 15 and 16, U-derived cells migrate from their initial ventral locations to their final destinations within the dorsal and central parts of the spinal cord. These cells migrate in a direction perpendicular to the radial pathways utilized by most other neurons. To determine the structural and/or molecular cues that guide this unique migration, Dr. Phelps will attempt to block the migration experimentally. Dr. Phelps uses an organotypic slice culture system that has been shown to support normal migration of U-derived cells and maintains relatively normal intercellular relationships between neurons and their environment, yet is accessible to both chemical and surgical manipulation, which would not be possible the whole embryo. A variety of different experiments are designed to disrupt this cell migration in culture. Results from these experiments will determine if the dorsal migration of the U-derived cells is guided by structural cues, such as early forming axons, and/or by molecular cues such as different types of adhesion molecules.

小行星9734550 菲尔普斯博士的早期职业生涯奖包含与发育神经生物学领域相关的教育和研究部分。 教育目标是建立一个基于互联网的多媒体学习系统，为一个新的本科课程，在发展神经生物学，与甘比尔博士和同事在加州大学洛杉矶分校的生物成像研究所的克伦普。 在神经系统发育过程中，发生了许多复杂的组织运动，这些运动对于学生来说很难使用教科书中的典型二维绘图来理解，但是当以三维动画呈现时，它们就变得生动起来。 设计彩色动画来描绘这些复杂的组织运动是这个项目的一个重要目标。 另一个重点是纳入在发育神经生物学领域工作的科学家的数据。 例如，演示活神经元观察的延时视频显微镜将用于课堂演示，向学生介绍令人兴奋的新研究技术。 学生可以通过网络复习课堂演示文稿，并利用互动测验作为学习辅助工具。 这种评估工具将帮助学生，但也将为教师提供有关这种新的基于互联网的学习系统的有效性的信息。 研究部分的目的是确定什么结构和/或分子指导一组化学定义的神经元在早期脊髓发育过程中的独特迁移。 研究中的神经元被称为“U-衍生”细胞，使用乙酰胆碱作为其神经递质，可以通过免疫学和组织化学方法进行鉴定。 在胚胎的第15和16天之间，U-衍生的细胞从它们最初的腹侧位置迁移到它们在脊髓的背侧和中央部分内的最终目的地。 这些细胞在垂直于大多数其他神经元所使用的放射状通路的方向上迁移。 为了确定引导这种独特迁移的结构和/或分子线索，Phelps博士将尝试通过实验阻止迁移。 Phelps博士使用了一种器官型切片培养系统，该系统已被证明可以支持U源细胞的正常迁移，并保持神经元与其环境之间相对正常的细胞间关系，但可以进行化学和手术操作，这在整个胚胎中是不可能的。 设计了各种不同的实验来破坏培养物中的这种细胞迁移。 这些实验的结果将确定U衍生细胞的背侧迁移是否由结构线索（例如早期形成的轴突）和/或分子线索（例如不同类型的粘附分子）引导。