Phylogenetic Analysis of Fungal Reproductive Strategies Using Mating Type Genes

使用交配型基因进行真菌繁殖策略的系统发育分析

• 批准号: 9806935
• 负责人: B. Gillian Turgeon
• 金额: --
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-10-01 至 2002-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9806935&HistoricalAwards=false
• 关键词: Phylogenetic; Analysis; Fungal; Reproductive; Strategies

Turgeon DEB 9806935 Species of the ascomycete genus Cochliobolus and its allied genera have been chosen as the focus for this study for their array of reproductive strategies, their suitability for genetic and molecular technology, and the ability of certain species within the group to cause devastating diseases of important crops. Our goal is to understand the evolution of fungal reproductive strategies and, eventually, pathogenic capability. Results will be directly relevant to the assessment of fungal biodiversity, to the exploitation of fungi in bioprospecting programs, and to design of strategies for control of diseases caused by fungi. Fungi are favorable subjects for these studies because they are small, tractable in the laboratory, and have features which make them ideal for comparative studies of reproduction. Closely related fungi can be self sterile (requiring a partner for mating), self-fertile (no partner required), obligately sexual or obligately asexual. We will use DNA sequences for genes that regulate sexual development the mating type (MAT) genes to explore the evolution of reproductive strategies in sexual and asexual fungi. MAT genes hold great promise for tracing relationships among fungal groups because they have superior phylogenetic resolving power. We will determine: 1) If asexual fungi really are asexual or if the sexual state is just undiscovered, 2) if MAT genes can be used to delineate species, and 3) if MAT gene phylogenies, together with new information on sexuality and species boundaries, can be used to predict future changes in fungal pathogenicity, and possibly the evolution of new pathogenic races. In addition, phylogenetic information will guide identification of fungi producing metabolites with potential pharmaceutical application and ongoing investigations into the molecular mechanisms of fungal pathogenesis.

Turgeon DEB 9806935 子囊菌属 Cochliobolus 及其近缘属的物种因其一系列繁殖策略、它们对遗传和分子技术的适用性以及该组中某些物种引起重要作物毁灭性疾病的能力而被选为本研究的重点。我们的目标是了解真菌繁殖策略的演变以及最终的致病能力。结果将直接关系到真菌生物多样性的评估、生物勘探计划中真菌的开发以及真菌引起的疾病控制策略的设计。真菌是这些研究的有利对象，因为它们体积小，在实验室中易于处理，并且具有使其成为生殖比较研究的理想选择的特征。密切相关的真菌可以是自体不育（需要伴侣交配）、自体受精（不需要伴侣）、专性有性或专性无性。我们将使用调节性发育的基因——交配型（MAT）基因的DNA序列来探索有性和无性真菌繁殖策略的进化。 MAT 基因在追踪真菌类群之间的关系方面具有广阔的前景，因为它们具有卓越的系统发育解析能力。我们将确定：1）无性真菌是否真的是无性的，或者有性状态是否只是未被发现，2）MAT基因是否可以用于描述物种，3）MAT基因系统发育以及有关性和物种边界的新信息是否可以用于预测真菌致病性的未来变化，以及可能的新致病种族的进化。此外，系统发育信息将指导鉴定产生具有潜在药物应用的代谢物的真菌以及正在进行的真菌发病机制的分子机制研究。