Epigenetic Silencing of Nuclear Transgenes in Chlamydomonas
衣藻核转基因的表观遗传沉默
基本信息
- 批准号:9808473
- 负责人:
- 金额:$ 30.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-09-01 至 2002-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Cerutti 9808473It has recently been demonstrated in this laboratory that epigenetic transcriptional inactivation occurs in algae as well as in higher plants. In Chlamydomonas, two mechanisms, namely chromatin/nuclear domains and DNA methylation, are hypothesized to be the responsible mechanisms. In order to test these working hypotheses, this project has the following specific aims: 1) To isolate tagged mutants defective in transcription gene silencing, 2) To clone and characterize a tagged gene(s) involved in transcription repression, and 3) To map the chromosomal location of silenced and stable transgenic loci.The production of transgenic plants and animals is rapidly becoming routine, but the introduced genes are often unstably expressed, leading to the loss or reduced manifestation of newly acquired traits. This phenomenon of transgene silencing often results from a variety of epigenetic mechanisms, operating at both transcriptional and post-transcriptional levels. In most cases the underlying molecular processes have not been precisely identified. The results of this work could contribute to a greater understanding of epigenetic phenomena in eukaryotes, since preliminary data suggest that the molecular mechanisms might be evolutionarily conserved. The analysis of epigenetic gene silencing in Chlamydomonas can provide a genetic approach for understanding essential features of chromosomal/nuclear organization controlling gene expression. From a practical perspective, the results could contribute to the development of methods to overcome epigenetic silencing methodology, which could contribute to the generation of transgenic plants and animals.
本实验室最近证实,表观遗传转录失活发生在藻类和高等植物中。在衣原体中,两种机制,即染色质/核结构域和DNA甲基化,被假设为负责机制。为了检验这些工作假设,该项目有以下具体目标:1)分离转录基因沉默缺陷的标记突变体,2)克隆和表征涉及转录抑制的标记基因,和3)绘制沉默和稳定的转基因基因位点的染色体定位。但是导入的基因通常不稳定地表达,导致新获得的性状的丧失或减少的表现。这种转基因沉默现象通常是由多种表观遗传机制引起的,在转录和转录后水平上起作用。在大多数情况下,潜在的分子过程尚未被精确识别。这项工作的结果可能有助于更好地理解真核生物中的表观遗传现象,因为初步数据表明,分子机制可能是进化保守的。衣原体表观遗传基因沉默的分析可以提供一个遗传学的方法来了解控制基因表达的染色体/核组织的基本特征。从实践的角度来看,这些结果可能有助于开发克服表观遗传沉默方法的方法,这可能有助于转基因植物和动物的产生。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Heriberto Cerutti其他文献
クラミドモナスmiRNAを介した遺伝子発現抑制はseed配列の相同性のみで誘導される
衣藻 miRNA 介导的基因表达抑制仅由种子序列同源性诱导
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
山崎朋人;Heriberto Cerutti;大濱武 - 通讯作者:
大濱武
小分子RNAを介した翻訳抑制とRaptorの役割
小RNA介导的翻译抑制和Raptor的作用
- DOI:
- 发表时间:
2012 - 期刊:
- 影响因子:0
- 作者:
山崎朋人;Heriberto Cerutti;大濱武 - 通讯作者:
大濱武
MLK4-mediated phosphorylation of histone H3T3 promoters flowering by transcriptional silencing of FLC/MAF in Arabidopsis thaliana
拟南芥中 MLK4 介导的组蛋白 H3T3 启动子磷酸化通过转录沉默 FLC/MAF 开花
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
Zhen Wang;Junmei Kang;Juan Arm;o Casas-Mollano;Yongchao Dou;Shangang Jia;Qingchuan Yang;Chi Zhang;Heriberto Cerutti - 通讯作者:
Heriberto Cerutti
単細胞緑藻クラミドモナスを用いたRNAサイレンシング機構の解析
利用单细胞绿藻衣藻分析 RNA 沉默机制
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
山崎朋人;Heriberto Cerutti;大濱武 - 通讯作者:
大濱武
On the origin and functions of RNA-mediated silencing: from protists to man
- DOI:
10.1007/s00294-006-0078-x - 发表时间:
2006-05-12 - 期刊:
- 影响因子:1.600
- 作者:
Heriberto Cerutti;J. Armando Casas-Mollano - 通讯作者:
J. Armando Casas-Mollano
Heriberto Cerutti的其他文献
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{{ truncateString('Heriberto Cerutti', 18)}}的其他基金
Mechanisms of Small RNA-Mediated Silencing in Chlamydomonas
衣藻中小 RNA 介导的沉默机制
- 批准号:
2131783 - 财政年份:2022
- 资助金额:
$ 30.5万 - 项目类别:
Standard Grant
REU Site: Integrated Development of Bioenergy Systems
REU网站:生物能源系统综合开发
- 批准号:
2050574 - 财政年份:2021
- 资助金额:
$ 30.5万 - 项目类别:
Standard Grant
Mechanisms of small RNA-mediated translation repression in Chlamydomonas
衣藻中小 RNA 介导的翻译抑制机制
- 批准号:
1616863 - 财政年份:2016
- 资助金额:
$ 30.5万 - 项目类别:
Standard Grant
Small RNA-mediated Translation Repression in Chlamydomonas
衣藻中小 RNA 介导的翻译抑制
- 批准号:
1244576 - 财政年份:2013
- 资助金额:
$ 30.5万 - 项目类别:
Continuing Grant
Histone H3 Phosphorylation and Gene Silencing in Chlamydomonas and Arabidopsis
衣藻和拟南芥中的组蛋白 H3 磷酸化和基因沉默
- 批准号:
1052281 - 财政年份:2011
- 资助金额:
$ 30.5万 - 项目类别:
Continuing Grant
Histone Modifications and Transcriptional Silencing in Chlamydomonas
衣藻中的组蛋白修饰和转录沉默
- 批准号:
0544448 - 财政年份:2006
- 资助金额:
$ 30.5万 - 项目类别:
Continuing Grant
Transcriptional Gene Silencing in Chlamydomonas and Arabidopsis
衣藻和拟南芥中的转录基因沉默
- 批准号:
0131357 - 财政年份:2002
- 资助金额:
$ 30.5万 - 项目类别:
Continuing Grant
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