Orientation and Dynamics of Myosin Head Regulatory Domain

肌球蛋白头调节域的方向和动力学

• 批准号: 9808708
• 负责人: Piotr Fajer
• 金额: $ 41万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9808708&HistoricalAwards=false
• 关键词: Orientation; Dynamics; Myosin; Head; Regulatory

9808708 Fajer The long term goal of Dr. Fajer's research is to determine molecular events which transform chemical energy of ATP into mechanical energy of muscle. Although muscular action is limited to animals, it is likely that a similar mechanism underlies action of other biological motors involved in cellular motility, cytoplasmic transport, separation of chromosomes, and activities necessary for any survival of all biological organisms. As with any motor, biological or not, one can expect moving parts and changes in relative disposition of proteins involved. Therefore, the investigation of protein dynamics and their orientation is indispensable in the attempt to understand the mechanism of biological motors In this grant the PI proposes to combine the spectroscopic methods capable of measuring both dynamics and orientation of labeled proteins with the chemical approaches trapping the biochemical cycle of actomyosin in well defined states. Since the myosin head does not behave as a rigid body, the PI plans to develop new labeled sites in the regulatory domain of myosin complementing the behavior of the catalytic domain.

9808708 Fajer Fajer 博士研究的长期目标是确定将 ATP 化学能转化为肌肉机械能的分子事件。 尽管肌肉活动仅限于动物，但类似的机制可能是其他生物马达作用的基础，这些生物马达涉及细胞运动、细胞质运输、染色体分离以及所有生物有机体生存所必需的活动。 与任何运动一样，无论是否是生物运动，人们都可以预见到运动部件和所涉及蛋白质的相对配置的变化。 因此，蛋白质动力学及其方向的研究对于理解生物马达的机制是必不可少的。在这项资助中，PI 建议将能够测量标记蛋白质的动力学和方向的光谱方法与将肌动球蛋白的生化循环捕获在明确状态的化学方法结合起来。 由于肌球蛋白头部并不表现为刚体，因此 PI 计划在肌球蛋白调节域中开发新的标记位点，以补充催化域的行为。