Orientation and Dynamics of Myosin Head Regulatory Domain

9808708法耶尔博士研究的长期目标是确定将三磷酸腺苷的化学能转化为肌肉机械能的分子事件。虽然肌肉活动仅限于动物，但很可能在其他生物马达的作用下也有类似的机制，这些马达涉及细胞运动、细胞质运输、染色体分离以及所有生物有机体生存所必需的活动。就像任何马达一样，无论是生物的还是非生物的，人们都可以预料到运动的部分和涉及的蛋白质的相对处置的变化。因此，对蛋白质动力学及其取向的研究对于试图理解生物马达的机制是不可或缺的。在这项研究中，PI建议将能够测量标记蛋白质的动力学和取向的光谱方法与捕捉处于明确状态的肌动蛋白的生化循环的化学方法相结合。由于肌球蛋白头部的行为不像一个刚体，PI计划在肌球蛋白的调节结构域中开发新的标记位点，以补充催化结构域的行为。

9808708 Fajer The long term goal of Dr. Fajer's research is to determine molecular events which transform chemical energy of ATP into mechanical energy of muscle. Although muscular action is limited to animals, it is likely that a similar mechanism underlies action of other biological motors involved in cellular motility, cytoplasmic transport, separation of chromosomes, and activities necessary for any survival of all biological organisms. As with any motor, biological or not, one can expect moving parts and changes in relative disposition of proteins involved. Therefore, the investigation of protein dynamics and their orientation is indispensable in the attempt to understand the mechanism of biological motors In this grant the PI proposes to combine the spectroscopic methods capable of measuring both dynamics and orientation of labeled proteins with the chemical approaches trapping the biochemical cycle of actomyosin in well defined states. Since the myosin head does not behave as a rigid body, the PI plans to develop new labeled sites in the regulatory domain of myosin complementing the behavior of the catalytic domain.