Orientation and Dynamics of Myosin Head Regulatory Domain

[808708]费杰博士研究的长期目标是确定将ATP的化学能转化为肌肉的机械能的分子事件。虽然肌肉运动仅限于动物，但很可能在其他生物运动的基础上也存在类似的机制，这些运动涉及细胞运动、细胞质运输、染色体分离以及所有生物有机体生存所必需的活动。与任何运动一样，无论是生物的还是非生物的，我们都可以期待运动部件和相关蛋白质相对配置的变化。因此，在试图理解生物马达的机制中，蛋白质动力学及其定向的研究是必不可少的。在这项资助中，PI建议将能够测量标记蛋白质的动力学和定向的光谱方法与捕获肌动球蛋白在明确状态下的生化循环的化学方法结合起来。由于肌凝蛋白头部的行为不像刚体，因此PI计划在肌凝蛋白的调节区域开发新的标记位点，以补充催化区域的行为。

9808708 Fajer The long term goal of Dr. Fajer's research is to determine molecular events which transform chemical energy of ATP into mechanical energy of muscle. Although muscular action is limited to animals, it is likely that a similar mechanism underlies action of other biological motors involved in cellular motility, cytoplasmic transport, separation of chromosomes, and activities necessary for any survival of all biological organisms. As with any motor, biological or not, one can expect moving parts and changes in relative disposition of proteins involved. Therefore, the investigation of protein dynamics and their orientation is indispensable in the attempt to understand the mechanism of biological motors In this grant the PI proposes to combine the spectroscopic methods capable of measuring both dynamics and orientation of labeled proteins with the chemical approaches trapping the biochemical cycle of actomyosin in well defined states. Since the myosin head does not behave as a rigid body, the PI plans to develop new labeled sites in the regulatory domain of myosin complementing the behavior of the catalytic domain.