RUI: Mechanisms of Activity-Induced Circadian Clock Resetting

摘要IBN 9808866是一种生物钟，它控制着许多身体功能和行为的日常节奏，可以通过剧烈运动来重新设定。时钟是否重置取决于一天中的时间和锻炼的强度。本项目将重点研究这种反应背后的行为和神经机制。由于不是所有的个体都表现出这种反应，该项目的第一部分将制定标准，以预测哪些动物表现出活动诱导的重启。下一步，将进行一项人工选择研究，以创造一系列丰富的表现出重置的动物的仓鼠。这条丰富的线将被用来绘制在重置期间激活的大脑区域。随后将进行实验，检查GABA的参与情况，GABA是大脑的一部分--膝状体-下丘脑束--的一种神经递质，此前曾被认为与活动诱导的重启有关。这项研究的结果将加深我们对哺乳动物大脑中时钟重置过程的理解。此外，本科生参与这个项目将为他们提供宝贵的实践学习经验，这些经验将补充和扩展他们的标准课程工作。

LAY ABSTRACT IBN 9808866 The circadian clock that controls daily rhythms of many bodily functions and behaviors can be reset in by vigorous exercise. Whether or not the clocks reset depends on the time of day and intensity of the exercise. This project will focus on the behavioral and neural mechanisms underlying this response. Because not all individuals show the response, the first part of the project will develop criteria to predict which animals show activity-induced resetting. Next, an artificial selection study will be conducted to create a line of hamsters enriched with animals that show resetting. This enriched line will then be used to map regions of the brain that are activated during resetting. This will be followed by experiments that examine the involvement of GABA, a neurotransmitter in a part of the brain - the geniculohypothalamic tract - that has previously been implicated in activity-induced resetting. The results of this study will enhance our understanding of the process of clock resetting in the mammalian brain. Furthermore, the participation of undergraduate students in this project will provide them with valuable hands-on learning experiences that will complement and extend their standard course work.