Mechanistic Studies on Dinuclear Hydrolytic Catalysts

双核水解催化剂的机理研究

• 批准号: 9816487
• 负责人: Richard Holz
• 金额: $ 30.6万
• 依托单位: Utah State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9816487&HistoricalAwards=false
• 关键词: Mechanistic; Studies; Dinuclear; Hydrolytic; Catalysts

Dr. Richard Holz, Department of Chemistry, Utah State University is supported by the Inorganic, Bioinorganic and Organometallic Chemistry Program of the Chemistry Division for an investigation of hydrolytic reactions catalyzed by dinuclear metal ion clusters. Specifically, Dr. Holz will elucidate the pathways of these reactions which are catalyzed by AAP (an aminopeptidase enzyme) with homo- and hetero-dinuclear metal clusters using a variety of spectroscopic and physical characterization techniques among which is time-resolved crystallography. The particular system chosen will serve both as a 'model" system and as a "real" system; this duality represents a truly state-of-the-art approach.The significance of this work is to gain an understanding of how di- and tri-nuclear metallohydrogenase enzymes hydrolyze such highly relevant biological molecules as DNA, RNA, phospholipids and polypeptides. The study will also provide an answer as to why the nickel- and copper-AAP enzymes are hyperactive relative to the zinc-AAP enzyme. This will be of interest to the biological and medical community. Graduate students and post-doctoral associates will receive an excellent training in bioinorganic chemistry.

犹他州州立大学化学系理查德·霍尔茨博士受化学部无机、生物无机和有机金属化学项目的支持，研究双核金属离子簇催化的水解反应。具体地说，Holz博士将使用各种光谱和物理表征技术阐明AAP(一种氨基肽酶)与同双核和异双核金属簇催化的这些反应的途径，其中包括时间分辨结晶学。这项工作的意义在于了解二核和三核金属氢酶是如何分解高度相关的生物分子，如DNA、RNA、磷脂和多肽。这项研究还将提供为什么镍和铜的AAP酶相对于锌的AAP酶的活性过高的答案。这将引起生物和医学界的兴趣。研究生和博士后将接受良好的生物无机化学培训。