NSF/EPA TSE: Novel Compressed Solvent Extraction Processes for Enhanced Biomass Conversion by Thermophilic Bacteria

NSF/EPA TSE：用于增强嗜热细菌生物质转化的新型压缩溶剂萃取工艺

• 批准号: 9817069
• 负责人: Barbara Knutson
• 金额: $ 34.44万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-15 至 2003-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9817069&HistoricalAwards=false
• 关键词: NSF; EPA; TSE; Novel; Compressed

The overall goal of the proposed work is to demonstrate the feasibility of extractive fermentation of volatile products using a compressed or supercritical solvent. The project will focus on the efficient continuous recovery of ethanol from biomass fermentation by the fibrolytic thermophilic bacteria, Clostridium thermocellum. This organism is capable of producing considerable amounts of ethanol directly from fibrous biomass, but is inhibited by the presence of relatively dilute ethanol. The Principal Invertigators' (PIs) hypthesis is that a compressed solvent is capable of efficiently extracting product so as to reduce product inhibition and thereby increase the fermentation rate and yield. Supercritical fluid extractive fermentation would have the advantages of gas-like mass transfer properties, solvent strength tunability, and ease of solvent regeneration and product recovery through depressurization. The specific objectives are to: (i) characterize effects of compressed solvents on the growth and metabolism of Clostridium thermocellum in the presence of soluble and insoluble carbohydrates; (ii) demonstrate in situ ethanol removal using a compressed fluid and evaluate the metabolic activity of Clostridium thermocellum as a function of extraction conditions; and (iii) develop a mathematicl model which will facilitate the design and analysis of compressed extractive fermentation processes. This award is made by NSF as part ofthe joint NSF/EPA Program on Technologies for a SustainableEnvironment.

拟议的工作的总体目标是证明挥发性产品的提取发酵使用压缩或超临界溶剂的可行性。 该项目将侧重于通过纤维分解嗜热细菌热纤梭菌从生物质发酵中高效连续回收乙醇。 这种生物能够直接从纤维生物质中产生大量的乙醇，但受到相对稀的乙醇的抑制。 主要研究者（PI）的假设是压缩的溶剂能够有效地提取产物，以减少产物抑制，从而提高发酵速率和产率。 超临界流体萃取发酵具有类似气体的传质特性、溶剂强度可调性、易于溶剂再生和通过减压回收产物等优点。 具体目标是：（i）表征在可溶性和不溶性碳水化合物存在下压缩溶剂对热纤梭菌生长和代谢的影响;（ii）证明使用压缩流体原位去除乙醇，并评估热纤梭菌作为提取条件的函数的代谢活性;和（iii）开发一个简化的模型，这将有助于压缩提取发酵过程的设计和分析。 该奖项是由NSF作为NSF/EPA可持续环境技术联合计划的一部分。