Biofilm Control Agents for Industry - ERC Connectivity Initiative

工业生物膜控制剂 - ERC Connectivity Initiative

• 批准号: 9817565
• 负责人: John Costerton
• 金额: $ 39.95万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9817565&HistoricalAwards=false
• 关键词: Biofilm; Control; Agents; Industry; ERC

The design of this project builds on the strengths of the Center for Biofilm Engineering (CBE - Montana State University) and the Center for Marine Biofouling and Bio-Inovation (BMBB - University of New South Wales, Sydney, Australia). As the CBE began to discover the structural complexity and architectural sophistication of biofilms, it became obvious that some form of cell-cell signaling must be operative in these biofilms. The CBE is to build a Biofilm Behavior Screenging Unit (BBSU) that will use confocal scanning microscopy (CSLM) and state-of-the-art image analysis to detect the effects of putative biofilm control molecules on biofilm formation and detachment. The CMBB discovered that some marine plants are protected from biofilm formation on their surfaces by a class of chemical compounds (furanones) that resemble the cell-cell signals (acyl-homoerine lactones - AHLs) used by bacteria for "quorum sensing". Both Centers will continue to determine the effects of natural AHLs and AHL antagonists on biofilm formation and detachmment and will cooperate in the selection of chemically stable, economical, nontoxic AHLs and AHL-like molecules that will serve industry as environmentally benign biofilm control agents.

该项目的设计建立在生物膜工程中心（CBE -蒙大拿州立大学）和海洋生物污染和生物创新中心（BMBB -新南威尔士大学，澳大利亚悉尼）的优势之上。随着CBE开始发现生物膜的结构复杂性和结构复杂性，很明显，在这些生物膜中一定有某种形式的细胞-细胞信号传导。CBE将建立一个生物膜行为筛选单元（BBSU），该单元将使用共聚焦扫描显微镜（CSLM）和最先进的图像分析来检测假定的生物膜控制分子对生物膜形成和脱离的影响。CMBB发现，一些海洋植物被一类类似于细菌用于“群体感应”的细胞-细胞信号（酰基-同质内酯- AHLs）的化合物（呋喃酮）保护，避免在其表面形成生物膜。两个中心将继续确定天然AHL和AHL拮抗剂对生物膜形成和分离的影响，并将合作选择化学稳定、经济、无毒的AHL和类AHL分子，这些分子将作为环境友好的生物膜控制剂服务于工业。