Experimental Coevolution between C. Elegans and B. Thuringiensis CLUSTER: "Experimental Evolution and Natural Variation of Bacillus-Invertebrate Interactions"

线虫和苏云金芽孢杆菌簇之间的实验协同进化：“芽孢杆菌-无脊椎动物相互作用的实验进化和自然变异”

• 批准号: 129842315
• 负责人: Professor Dr. Hinrich Schulenburg
• 金额: --
• 依托单位: Max-Planck-Institut für Evolutionsbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/129842315?language=en
• 关键词: Experimental; Coevolution; between; C.; Elegans

The coevolution between host and parasite is believed to associate with very high selection dynamics that are of prime importance for the evolution of organisms and biological systems. Despite numerous theoretical studies, conclusive empirical evidence for the complexity of evolutionary effects and the underlying genetic processes is still scarce. The aim of this project is to provide an experimental test of the consequences of host-parasite coevolution at both phenotypic and genomic levels. During the current funding period, we have used evolution experiments to generate strains of hosts (the nematode Caenorhabditis elegans) and parasites (its microparasite Bacillus thuringiensis) with known histories of either coevolution or one-sided adaptation. The imposed differences in evolutionary history indeed caused significant variation in parasite virulence, host resistance and also other life-history traits. During the proposed funding period, we will focus on understanding the genetic basis of the observed phenotypic changes, using a combination of population genomic analysis, quantitative trait locus (QTL) analysis of a new population of recombinant inbred lines (RILs), and the functional genetic analysis of identified candidate genes. We will further link our empirical data to theoretical models to refine our understanding of the dynamics of these interactions. This project is proposed as part of the Bacillus-Invertebrate Cluster within the Priority Programme on Host-Parasite Coevolution.

宿主和寄生虫之间的协同进化被认为与非常高的选择动力学有关，这对于生物和生物系统的进化至关重要。尽管有大量的理论研究，进化效应和潜在的遗传过程的复杂性的结论性的经验证据仍然是稀缺的。该项目的目的是提供一个实验测试的结果宿主寄生虫共同进化的表型和基因组水平。在目前的资助期内，我们已经使用进化实验来产生具有已知的共同进化或单侧适应历史的宿主（线虫秀丽隐杆线虫）和寄生虫（其微寄生虫苏云金芽孢杆菌）菌株。在进化史上的差异确实造成了寄生虫毒力，宿主抗性和其他生活史特征的显着变化。在拟议的资助期内，我们将重点了解观察到的表型变化的遗传基础，使用人口基因组分析，重组自交系（RILs）的新人口的数量性状位点（QTL）分析，并确定候选基因的功能遗传分析相结合。我们将进一步将我们的经验数据与理论模型联系起来，以完善我们对这些相互作用的动力学的理解。该项目是作为宿主-寄生虫共同进化优先方案内芽孢杆菌-无脊椎动物集群的一部分提出的。