An evolutionary perspective in innate immunity using the nematode Caenorhabditis elegans

使用线虫秀丽隐杆线虫研究先天免疫的进化观点

• 批准号: 5419517
• 负责人: Professor Dr. Hinrich Schulenburg
• 金额: --
• 依托单位: Arbeitsgruppe Evolutionsökologie und Genetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5419517?language=en
• 关键词: evolutionary; perspective; innate; immunity; using

Simple model organisms that are amenable to comprehensive experimental analysis show enormous potential to elucidate the molecular genetic architecture of complex traits and thus to enhance our understanding of these traits in other organisms, including humans. In the proposed project, we will exploit the advantages of the nematode Caenorhabditis elegans as an experimental system to study the molecular genetic basis of innate immunity. In particular, we will examine the induced immune response of two natural C. elegans strains towards the Gram-positive bacterium Bacillus thuringiensis. The microarray technology is employed in conjunction with Northern analysis for a first identification of induced gene expression. The exact function of candidate genes is subsequently studied by analysis of available mutant strains and by gene silencing using RNA interference. We expect that this approach will yield information about the conserved and variable components of the nematode's immune response. Considering that only little information is as yet available for lower invertebrates, including C. elegans, these results should provide novel insights into the evolution and function of the animal innate immune system.

简单的模式生物，适合全面的实验分析显示出巨大的潜力，阐明复杂性状的分子遗传结构，从而提高我们对这些性状在其他生物体，包括人类的理解。本研究将利用秀丽隐杆线虫作为实验系统，研究天然免疫的分子遗传基础。特别地，我们将检查两种天然C. elegans菌株对革兰氏阳性细菌苏云金芽孢杆菌。微阵列技术与北方分析结合使用，用于诱导基因表达的首次鉴定。随后通过分析可用的突变株和通过使用RNA干扰的基因沉默来研究候选基因的确切功能。我们期望这种方法将产生有关线虫免疫反应的保守和可变成分的信息。考虑到目前对包括C. elegans，这些结果应该提供新的见解的进化和动物先天免疫系统的功能。